600123 | Human IFN-gamma Clinisciences

Recombinant Active Protein

Cat. No.

600123

Unit

0.1 mg

Description

Interferon gamma (IFN-gamma) is the major interferon produced by stimulated lymphocytes. IFN-gamma is structurally different from type I interferon and its major activity is immunoregulation. It has been implicated in the expression of class II histocompatibility antigens in cells that do not normally produce them, leading to autoimmune disease. IFN-gamma is mainly produced by T-cells and natural killer cells activated by antigens, mitogens, or alloantigens. It is produced by lymphocytes expressing the surface antigens CD4 and CD8. The synthesis of IFN-gamma is induced by Interleukin-2, FGF-basic, and EGF. Recombinant Human IFN-gamma produced in E. coli is a single non-glycosylated polypeptide chain containing 144 amino acids with a MW of 16,879 Da.

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Datasheet

Format

Each vial contains 100 ug of lyophilized protein. Reconstitute with 1.0 ml sterile deionized water for a final concentration of 0.1 mg/ml.

Alternate Names

interferon gamma; IFN-gamma; immune interferon; IFNG

Accession No.

P01579

16879 Da

Sequence

MQDPYVKEAE NLKKYFNAGH SDVADNGTLF LGILKNWKEE SDRKIMQSQI VSFYFKLFKN FKDDQSIQKS VETIKEDMNV KFFNSNKKKR DDFEKLTNYS VTDLNVQRKA IHELIQVMAE LSPAAKTGKR KRSQMLFQGR RASQ

Source

E. coli. Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.

Purity

Purity > 95% as determined by analysis by RP-HPLC and reducing and non-reducing SDS-PAGE. Protein Content determined by UV spectroscopy at 280 nm and analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.

Application Notes

Recombinant Interferon gamma is fully biologically active when compared to standard. The specific activity as determined in a viral resistance assay is less then 0.05 ng/ml.

Storage

Store at -20°C. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20°C. We recommend to add a carrier protein (0.1% HSA or BSA) for long term storage.

Images

For research use only, not for diagnostic or therapeutic procedures.

Human IFN-gamma

Referência 600123

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Marca : Abbiotec

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