Human IgD mouse monoclonal antibody, clone NI 158, Biotin
Specifications
Product Data
Clone Name
NI 158
Application
ELISA, IF, IP, WB
Recommended Dilution
To identify the presence of IgD in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, immunoperoxidase staining of cytoplasmic IgD, and immunoblotting. As a second step an avidin or streptavidin conjugate of the customer’s choice have to be used. General Recommended Dilutions: Histochemical Use: 1/50-1/200 ELISA: from 1/500 upwards. Western blot: from 1/1000 upwards.
Reactivity
Human
Antibody Host
Mouse
Isotype
IgG1
Clonality
Monoclonal
Immunogen
Highly purified polyclonal IgD isolated from human serum.
Specificity
The reactivity of the antiserum is restricted to an isotype specific determinant of the IgD molecule as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immunoperoxidase staining of cytoplasmic Ig. The antiserum does not react with any other component of the human Ig system or any other human plasma protein as tested. This antiserum has not been tested for cross-reactivity with other species.
Buffer
PBS, pH 7.2 without preservatives or foreign proteins. Label: Biotin State: Lyophilized purified Ig fraction Molar radio: Biotin/IgG ~5.0
Reconstitution Method
Restore by adding 0.5 ml sterile distilled water.
Concentration
0.4 mg/ml
Conjugation
Biotin
Storage
Store lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer. Avoid Repeated thawing and freezing.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
