Human IgG2 (Fc specific) mouse monoclonal antibody, clone NI 25-1 (HP 6207), Biotin
Specifications
Product Data
Clone Name
NI 25-1 (HP 6207)
Application
ELISA, IF, IP, WB
Recommended Dilution
To identify the presence of IgG2 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, immunoperoxidase staining of cytoplasmic IgG2, and immunoblotting. As a second step an avidin or streptavidin conjugate of the customer’s choice have to be used. General Recommended Dilutions: Histochemical Use: 1/50-1/250. ELISA: from 1/100 upwards. Western blot: from 1/500 upwards.
Reactivity
Human
Antibody Host
Mouse
Isotype
IgG1
Clonality
Monoclonal
Immunogen
Highly purified monoclonal IgG2 isolated from pooled Human serum.
Specificity
The reactivity of the antiserum is restricted to a subclass specific determinant on the Fc portion of the IgG2 molecule as tested in direct binding enzyme Immunoassay, Immunoblotting, Immunoprecipitation and direct immunoperoxidase staining of cytoplasmic Ig. Cross-Reactivity: The antiserum does not react with any other component of the Human Ig system or any other Human plasma protein as tested. This antiserum has not been tested for cross-reactivity with other species.
Buffer
PBS, pH 7.2 without preservatives or foreign proteins Label: Biotin State: Lyophilized purified Ig fraction Molar radio: Biotin/IgG ~4.2
Reconstitution Method
Restore by adding 0.5 ml sterile distilled water
Concentration
0.4 mg/ml
Conjugation
Biotin
Storage
Store lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer. Avoid Repeated thawing and freezing.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
