Human LRRK2 (Leucine Rich Repeat Kinase 2) Sandwich ELISA Kit

Storage Instruction:	Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time:	4.5 hrs

Gene Symbol:	LRRK2
Gene ID:	120892
Uniprot ID:	LRRK2_HUMAN
Sample Type:	tissue homogenates, cell lysates or other biological fluids.

Tissue Specificity	Expressed in pyramidal neurons in all cortical laminae of the visual cortex, in neurons of the substantia nigra pars compacta and caudate putamen (at protein level). Expressed in neutrophils (at protein level). Expressed in the brain. Expressed throughout the adult brain, but at a lower level than in heart and liver. Also expressed in placenta, lung, skeletal muscle, kidney and pancreas. In the brain, expressed in the cerebellum, cerebral cortex, medulla, spinal cord occipital pole, frontal lobe, temporal lobe and putamen. Expression is particularly high in brain dopaminoceptive areas.
Post Translational Modifications	Autophosphorylated. Phosphorylation of Ser-910 and either Ser-935 or Ser-1444 facilitates interaction with YWHAG. Phosphorylation of Ser-910 and/or Ser-935 facilitates interaction with SFN. Ubiquitinated by TRIM1.undergoes 'Lys-48'-linked polyubiquitination leading to proteasomal degradation.
Function	Serine/threonine-protein kinase which phosphorylates a broad range of proteins involved in multiple processes such as neuronal plasticity, innate immunity, autophagy, and vesicle trafficking. Is a key regulator of RAB GTPases by regulating the GTP/GDP exchange and interaction partners of RABs through phosphorylation. Phosphorylates RAB3A, RAB3B, RAB3C, RAB3D, RAB5A, RAB5B, RAB5C, RAB8A, RAB8B, RAB10, RAB12, RAB35, and RAB43. Regulates the RAB3IP-catalyzed GDP/GTP exchange for RAB8A through the phosphorylation of 'Thr-72' on RAB8A. Inhibits the interaction between RAB8A and GDI1 and/or GDI2 by phosphorylating 'Thr-72' on RAB8A. Regulates primary ciliogenesis through phosphorylation of RAB8A and RAB10, which promotes SHH signaling in the brain. Together with RAB29, plays a role in the retrograde trafficking pathway for recycling proteins, such as mannose-6-phosphate receptor (M6PR), between lysosomes and the Golgi apparatus in a retromer-dependent manner. Regulates neuronal process morphology in the intact central nervous system (CNS). Plays a role in synaptic vesicle trafficking. Plays an important role in recruiting SEC16A to endoplasmic reticulum exit sites (ERES) and in regulating ER to Golgi vesicle-mediated transport and ERES organization. Positively regulates autophagy through a calcium-dependent activation of the CaMKK/AMPK signaling pathway. The process involves activation of nicotinic acid adenine dinucleotide phosphate (NAADP) receptors, increase in lysosomal pH, and calcium release from lysosomes. Phosphorylates PRDX3. By phosphorylating APP on 'Thr-743', which promotes the production and the nuclear translocation of the APP intracellular domain (AICD), regulates dopaminergic neuron apoptosis. Acts as a positive regulator of innate immunity by mediating phosphorylation of RIPK2 downstream of NOD1 and NOD2, thereby enhancing RIPK2 activation. Independent of its kinase activity, inhibits the proteasomal degradation of MAPT, thus promoting MAPT oligomerization and secretion. In addition, has GTPase activity via its Roc domain which regulates LRRK2 kinase activity.
Protein Name	Leucine-Rich Repeat Serine/Threonine-Protein Kinase 2 Dardarin
Database Links	Reactome: R-HSA-8857538
Cellular Localisation	Cytoplasmic Vesicle Perikaryon Golgi Apparatus Membrane Peripheral Membrane Protein Cell Projection Axon Dendrite Endoplasmic Reticulum Membrane Secretory Vesicle Synaptic Vesicle Membrane Endosome Lysosome Mitochondrion Outer Membrane Cytoplasm Cytoskeleton Colocalized With Rab29 Along Tubular Structures Emerging From Golgi Apparatus Localizes To Endoplasmic Reticulum Exit Sites (Eres) Also Known As Transitional Endoplasmic Reticulum (Ter)
Alternative ELISA Names	Leucine-Rich Repeat Serine/Threonine-Protein Kinase 2 ELISA kit Dardarin ELISA kit LRRK2 ELISA kit PARK8 ELISA kit
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Information sourced from Uniprot.org

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