K1031-100 | HyperPFU™ high-fidelity DNA polymerase Clinisciences

Product Citation

1. Qing Wang, Zhiyi He, et al. "Polyphyllin B inhibited STAT3/NCOA4 pathway and restored gut microbiota to ameliorate lung tissue injury in cigarette smoke-induced mice." BMC Biotechnol. 2024 Mar 8;24(1):13. PMID: 38459479
2. Ruoxia Wu, Jiaqing Xiong, et al. "Quercetin/Anti-PD-1 Antibody Combination Therapy Regulates the Gut Microbiota, Impacts Macrophage Immunity and Reshapes the Hepatocellular Carcinoma Tumor Microenvironment." Front Biosci (Landmark Ed). 2023 Dec 1;28(12):327. PMID: 38179731
3. Pablo Rodríguez-Fernández, Laure Botella, et al. "MptpB Inhibitor Improves the Action of Antibiotics against Mycobacterium tuberculosis and Nontuberculous Mycobacterium avium Infections." ACS Infect Dis. 2023 Dec 12. PMID: 38085851
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5. Man Luo, Yuanyuan Chen, et al. "E. coli Nissle 1917 ameliorates mitochondrial injury of granulosa cells in polycystic ovary syndrome through promoting gut immune factor IL-22 via gut microbiota and microbial metabolism." Front Immunol. 2023 May 19:14:1137089. PMID: 37275915
6. Li Zhang, Junjuan Lu, et al. "Akkermansia muciniphilaAmeliorates Lung Injury in Smoke-Induced COPD Mice by IL-17 and Autophagy." Cellular Microbiology. 15 March 2023.
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Description

HyperPFU™ high-fidelity DNA polymerase is composed of a DNA-binding domain fused to a Pyrococcus-like proofreading polymerase. Because of this, it produces PCR products with very high speed and accuracy. Also, HyperPFU™ high-fidelity DNA polymerase is tolerant of various inhibitors, allowing for robust amplification with minimal optimization. HyperPFU™ high-fidelity DNA polymerases is an ideal choice for cloning and can be used for long or difficult (GC-rich) amplicons. It becomes the choice for even demanding applications like massively parallel high-throughput sequencing of whole genomes.
HyperPFU™ high-fidelity DNA polymerase is important for applications in which the DNA sequence needs to be correct after amplification. Its unique structure, a novel Pyrococcus-like enzyme fused with a processivity-enhancing domain, increases fidelity and speed. HyperPFU™ high-fidelity DNA polymerases is an ideal choice for cloning and can be used for long or difficult amplicons. With an error rate >50-fold lower than that of Taq DNA Polymerase and 6-fold lower than that of Pyrococcus furiosus DNA Polymerase (1), it is one of the most accurate thermostable polymerases available. It possesses 5´→ 3´ polymerase activity, 3´→ 5´ exonuclease activity and will generate blunt-ended products.
HyperPFU™ high-fidelity DNA polymerases is supplied with standard 5X HF Buffer, which can be used for complex or GC-rich templates.

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HyperPFU™ high-fidelity DNA polymerase

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