K1074-100 | HyperScript™ RT SuperMix for qPCR Clinisciences

Product Citation

1. Kai Zhang, Hongkun Hu, et al. "The bio-functionalized membrane loaded with Ta/WH nanoparticles promote bone regeneration through neurovascular coupling." Colloids Surf B Biointerfaces. 2023 Aug 7;230:113506. PMID: 37572400

Description

HyperScript™ RT SuperMix for qPCR is the premixed solution for reverse transcription reaction, based on HyperScript™ Reverse Transcriptase (Cat. No. K1071). HyperScript™ Reverse Transcriptase is a new enzyme obtained through genetic engineering based on M-MLV (RNase H-) Reverse Transcriptase. In comparison, HyperScript™ Reverse Transcriptase reduces RNase H activity and increases thermal stability. HyperScript™ Reverse Transcriptase can withstand higher reaction temperatures and is suitable for reverse transcription of RNA templates with complex secondary structures.

HyperScript™ RT SuperMix for qPCR is suitable for two-step qRT-PCR detection ,5X RT SuperMix contains all the components required for reverse transcription, and the reaction can be carried out quickly by only adding template RNA and RNase Free ddH₂O. the volume of the RNA template can be added up to 80% of the total volume, which is very suitable for the reverse transcription reaction of the low concentration RNA template. 5X RT SuperMix will not freeze at -20°C, easy to use. This product is specially optimized for qPCR. the proportionally optimized Oligo (dt)₂₃vn primer mix/random primers enables cDNA synthesis to start from various regions of the RNA transcript with the same reverse transcription efficiency, maximizing the authenticity and repeatability of the qPCR results. The reverse transcription products are suitable for qPCR by Green and probe methods. The corresponding reagents can be selected for high performance gene expression analysis according to the experimental purpose.

Quality Control

Protocol

Related Biological Data

Related Biological Data

Components and Storage

Components	50 rxns (20 μL reaction)	100 rxns (20 μL reaction)
RNase Free ddH₂O	1 mL	1 mL x 2
5X RT SuperMix	200 μL	400 μL
5X No RT control Mix	20 μL	40 μL
Store the components at -20°C.

Features

5X RT SuperMix contains all the ingredients required for reverse transcription and is easy to operate;

The optimized ratio of Oligo (dT)₂₃VN primers to random primers can ensure the authenticity and repeatability of qPCR results to the greatest extent;

Efficient Reverse Transcriptase for RNA templates containing secondary structures;

Detects template starting amounts as low as 1 pg;

Oligo (dT)₂₃VN primers have stronger template anchoring ability and higher reverse transcription efficiency than Oligo (dT)₁₈.

Storage

Store the components at -20°C.

HyperScript™ RT SuperMix for qPCR

Referência K1074-100

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Product Citation

Description

Quality Control

Related Biological Data

Related Biological Data

Related Biological Data

Related Biological Data

Components and Storage

Features

Storage

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