IgG3 (AP)
Referência I1904-85J-1mg
Tamanho : 1mg
Marca : US Biological
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I1904-85J Rabbit Anti-IgG3 (AP)
Clone Type
PolyclonalHost
rabbitSource
mouseIsotype
IgGGrade
Affinity PurifiedApplications
DB E IHC WBCrossreactivity
MoShipping Temp
Blue IceStorage Temp
4°C Do Not FreezeApplications: |Suitable for Immunoblotting (Western or Dot Blot), ELISA and immunohistochemistry. Other applications not tested.||Recommended Dilutions: |ELISA: 1:2000-1:4000 Use pNPP p-nitrophenyl phosphate NPP-10 as a substrate for 30 minutes at RT.|Optimal dilution determined by the researcher. ||Label: |Alkaline Phosphatase (Calf Intestine) (MW 140kD)||Storage and Stability:|May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Applications
Product Type: Pab|Isotype: IgG|Host: rabbit|Source: mouse|Concentration: ~1mg/ml (UV280)|Form: Supplied as a liquid in TBS, 1mM magnesium chloride, 0.1mM zinc chloride, pH 8.0, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide, 50% glycerol. Labeled with alkaline phosphotase (AP).|Purity: Purified by immunoaffinity chromatography followed by solid phase adsorptions to remove any unwanted reactivities.|Immunogen: Mouse IgG3 heavy chain|Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-alkaline phosphatase (calf intestine) and anti-rabbit serum. Specificity was confirmed by ELISA at less than 1% crossreactivity against other mouse heavy or light chain isotypes.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Mouse IgG3 heavy chain
Form
Supplied as a liquid in TBS, 1mM magnesium chloride, 0.1mM zinc chloride, pH 8.0, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide, 50% glycerol. Labeled with alkaline phosphotase (AP).
Purity
Purified by immunoaffinity chromatography followed by solid phase adsorptions to remove any unwanted reactivities.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-alkaline phosphatase (calf intestine) and anti-rabbit serum. Specificity was confirmed by ELISA at less than 1% crossreactivity against other mouse heavy or light chain isotypes.