IL17A ELISA Kit (Rat)

Referência OKEH00104

Tamanho : 96Wells

Marca : Aviva Systems Biology

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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationELISA-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology IL17A ELISA Kit (Rat) (OKEH00104) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IL17A has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IL17A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IL17A captured in well.
ELISA Kit Range15.6-1000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 94%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.1% (n = 20)
Mean Inter-assay CV%: < 7.7% (n = 20)
ELISA Kit Component
ComponentAmount
IL17A Microplate96 Wells (12 x 8 Well strips)
IL17A Lyophilized Standard2
100X Biotinylated IL17A Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity7.86 pg/mL
SpecificityNatural and recombinant Rat Interleukin-17A
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolIL17A
Gene Full Nameinterleukin 17A
Alias SymbolsCytotoxic T-lymphocyte-associated antigen 8.
NCBI Gene Id301289
Protein NameQ61453
Description of TargetInterleukin-17A
Uniprot IDhttps://www.uniprot.org/uniprot/Q61453
Protein Accession #NP_001100367
Nucleotide Accession #NM_001106897
Protein Size (# AA)150
Molecular Weight17 kDa

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