iPSC Induction Enhancer, Thiazovivin (N-Benzyl-2-(pyrimidin-4-ylamino)thiazole-4-carboxamide, Induced Pluripotent Stem Cells Induction Enhancer, Rho Kinase Inhibitor XII)

A cell-permeable aminothiazolo-carboxamide compound that is reported to augment SB431542 and PD0325901 in enhancing human iPSCs (induced Pluripotent Stem Cells) generation from fibroblast cultures virally transfected with 4-TF (Oct44, Klf4, Sox2, and c-Myc). Simultaneous blockage of TGFb and MEK-ERK pathways by SB431542 (2uM) and PD0325901 (0.5uM) in addition to 4-TF-induction is shown to result in >50-fold improvement of iPSCs generation, while the inclusion of Thiazovivin (0.5uM) on top of SB431542 and PD0325901 is demonstrated to further improve the efficiency to an over-all of >200-fold improvement from 4-TF-induction alone.||Formula:|C15H13N5OS||Molecular Weight:|311.4||Solubility:|DMSO (100mg/ml)||Storage and Stability:|Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.