Images
Western Blot
Application

Western Blot

The whole cell lysate derived from m-CSF stimulated Jurket cells (Lane 1) or mouse spleen (Lane 2) were immune-probed by LSP1 (phospho S252) polyclonal antibody (Cat # PAB12633) at 1 : 500. An immunoreactive band is observed around ~ 52 KDa.

  • Specifications

    Product Description

    Rabbit polyclonal antibody raised against synthetic phosphopeptide of LSP1.

    Immunogen

    Synthetic phosphopeptide corresponding to residues surrounding S252 of human LSP1.

    Host

    Rabbit

    Theoretical MW (kDa)

    52

    Reactivity

    Human, Mouse

    Specificity

    This antibody recognizes ~52 KDa of human LSP1 at a phosphorylated serine 252. It does not recognize non-phosphorylated peptides confirmed by dot blot.

    Form

    Liquid

    Quality Control Testing

    Antibody Reactive Against Synthetic Peptide.

    Recommend Usage

    Western Blot (0.1-1 ug/mL)
    ELISA (0.01-0.1 ug/mL)
    Immunoprecipitation (2-5 ug/mL)
    Immunohistochemistry (2-5 ug/mL)
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In TBS, pH 7.2 (BSA, 10% Proclin300)

    Storage Instruction

    Store at 4°C. For long term storage store at -20°C or -80°C.
    Aliquot to avoid repeated freezing and thawing.

  • Applications

    Western Blot

    The whole cell lysate derived from m-CSF stimulated Jurket cells (Lane 1) or mouse spleen (Lane 2) were immune-probed by LSP1 (phospho S252) polyclonal antibody (Cat # PAB12633) at 1 : 500. An immunoreactive band is observed around ~ 52 KDa.

    Immunohistochemistry

    Immunoprecipitation

    Enzyme-linked Immunoabsorbent Assay

  • Gene Info — LSP1

    Entrez GeneID

    4046

    Gene Name

    LSP1

    Gene Alias

    WP34, pp52

    Gene Description

    lymphocyte-specific protein 1

    Omim ID

    153432

    Gene Ontology

    Hyperlink

    Gene Summary

    This gene encodes an intracellular F-actin binding protein. The protein is expressed in lymphocytes, neutrophils, macrophages, and endothelium and may regulate neutrophil motility, adhesion to fibrinogen matrix proteins, and transendothelial migration. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq

    Other Designations

    47 kDa actin binding protein|52 kDa phosphoprotein|F-actin binding and cytoskeleton associated protein|OTTHUMP00000014139|OTTHUMP00000069612|leufactin (leukocyte F-actin binding protein)|leukocyte-specific protein 1|lymphocyte-specific antigen WP34|lympho

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