K2068-100 assays | Magnesium Colorimetric Assay Kit Clinisciences

Background

Magnesium is the 11th most abundant element in the human body. Mg²⁺ is essential for all living cells where it is involved in facilitating the processing of biological polyphosphates like DNA, RNA, ATP and enzyme functions. Mg²⁺ is the metallic ion at the center of chlorophyll. Mg²⁺can be used as antacids and laxatives, and used to stabilize blood vessel spasm such as eclampsia and abnormal nerve excitation.

The Magnesium Colorimetric Assay Kit provides a simple and sensitive way for detection of magnesium concentration in various biological samples based on colorimetric method and the specific requirement of glycerol kinase for Mg²⁺. The enzyme linked reaction generates an intensely colored (λmax = 450 nm) product whose formation is proportional to Mg²⁺concentration. The colored product can be easily qualified using a microtiter plate reader or a spectrophotometer at 450 nm. The linear range is 2-15 nmoles with detection sensitivity ~ 40 µM.

Features & Properties

Features	Fast and convenient Simple procedure; takes less ~30 minutes The assay is sensitive, stable and high-throughput adaptable. The kit takes advantage of the specific requirement of glycerol kinase for Mg2+.
Shipping	Blue Ice
Storage Conditions	Store at +4°C.

Quality Control

Related Biological Data

Storage

Store at +4°C.

Overview

Kit components

Magnesium Assay Buffer Magnesium Developer Magnesium Enzyme Mix Magnesium Standard (150 nmol/µL)

Magnesium Colorimetric Assay Kit

Referência K2068-100 assays

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Marca : APExBIO Technology