MEL-CLS-2 growing culture
Referência 330283
Tamanho : 1cellcultureflask
Marca : CLS Cell Lines Service
MEL-CLS-2 Cells
General information
Description | This cell line was established as in vitro cell line from the melanoma xenograft of a primary melanoma by CLS in 1998. |
---|---|
Organism | Human |
Tissue | Human xenograft |
Disease | Melanoma |
Characteristics
Age | Unspecified |
---|---|
Gender | Male |
Ethnicity | Caucasian |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | MEL-CLS-2 (Cytion catalog number 300283) |
---|---|
Biosafety level | 1 |
Expression / Mutation
Protein expression | p53(+) |
---|---|
Tumorigenic | Yes, in nude mice |
Mutational profile | BRAF V600Emut |
Karyotype | Modal number 51, range 38-56 |
Handling
Culture Medium | DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a) |
---|---|
Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:3 to 1:4 is recommended |
Seeding density | 1 to 2 x 10^4 cells/cm^2 |
Fluid renewal | Every 3 days |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
|
Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
---|---|
STR profile | Amelogenin: x,y CSF1PO: 11,12 D13S317: 9,10 D16S539: 11 D5S818: 9,11 D7S820: 7,10 TH01: 9,9.3 TPOX: 8,9 vWA: 15,17 D3S1358: 15 D21S11: 29,30 D18S51: 12,17 Penta E: 7,11 Penta D: 9,13 D8S1179: 15 FGA: 23 |
HLA alleles | A*: 02:06:01, 20:02:01 B*: 01.01.1900 15:01, 02.01.1900 05:01 C*: 03:04:01, 04:01:01 DRB1*: 03:01:01, 04:01:01 DQA1*: 03:03:01, 05:01:01 DQB1*: 02:01:01, 03:01:01 DPB1*: 02:01, 04:01 E: 01:01, 01:03 |