Functionalized Polystyrene

Our carboxylate-modified polystyrene latex microspheres are suitable for the covalent immobilization of proteins, peptides, and nucleic acids. They are synthesized via emulsion polymerization, and are available in diameters up to ~20µm, with typical size CVs of 10%. Products are supplied as 10% solids suspension (w/w) in de-ionized water with surfactant and sodium azide.
 

Carboxyl Polystyrene

EDAC-mediated coupling is often used for the covalent immobilization of amine-terminated proteins, DNA, or other molecules on carboxyl-functionalized PS and P(S/DVB) microspheres. Carboxyl polymer microspheres are available as ~10% solids (w/w) aqueous suspensions in these standard amounts: 0.5g, 1.0g, 1.5g, and 5.0g. 
New to beads? See our PolyLink Protein Coupling Kit .

TechNote 205, Covalent Coupling
PDS 732, Polystyrene Microspheres

Catalog Number Nominal Diameter

Specification Range
PC02001 0.025µm 0.015 - 0.035µm
PC02002 0.050µm 0.040 - 0.060µm
PC02003 0.070µm 0.061 - 0.080µm
PC02004 0.100µm 0.090 - 0.110µm
PC02005 0.125µm 0.115 - 0.135µm
PC02006 0.150µm 0.140 - 0.160µm
PC02007 0.175µm 0.165 - 0.185µm
PC02008 0.200µm 0.190-0.210µm
PC02009 0.300µm 0.270-0.330µm
PC02010 0.350µm 0.340 - 0.360µm
PC02011 0.400µm 0.370 - 0.430µm
PC03001 0.500µm 0.470 - 0.530µm
PC03002 0.600µm 0.570 - 0.630µm
PC03003 0.800µm 0.770 - 0.830µm
PC03004 0.900µm 0.870 - 0.930µm
PC04001 1.0µm 0.95 - 1.05µm
PC05001 2.00µm 1.80 - 2.20µm
PC05002 2.50µm 2.30 - 2.70µm
PC05003 3.00µm 2.80 - 3.20µm
PC05004 4.00µm 3.80 - 4.20µm
PC05005 4.50µm 4.30 - 4.70µm
PC06001 5.00µm 4.80 - 5.20µm
PC06002 5.50µm 5.30 - 5.70µm
PC06003 6.00µm 5.80 - 6.20µm
PC06004 7.00µm 6.80 - 7.20µm
PC07001 10.00µm 9.50 - 10.50µm
PC07002 15.00µm 14.50µm - 15.50µm
PC07003 20.00µm 19.00µm - 21.00µm
PC08001 >25.µm >25.00µm
(Inquire)

References

Park, MK, Briles, DE, Nahm, MH (2000) A Latex Bead-Based Flow Cytometric Immunoassay Capable of Simultaneous Typing of Multiple Pneumococcal Serotypes (Multibead Assay) Clin Vaccine Immunol v7(3) 486-489

Blanchette C, Lacayo CI, Fischer NO, Hwang M, Thelen MP (2012) Enhanced Cellulose Degradation Using Cellulase-Nanosphere Complexes. PLoS ONE 7(8): e42116

Rodrigues OR, & Monard S, (2016) A rapid method to verify single-cell deposition setup for cell sorters. Cytometry 89: 594–600

Borque L, Bellod L, Rus A, Seco L, Galisteo-Gonzàles F (2000) Development and Validation of an Automated and Ultrasensitive Immunoturbidimetric Assay for C-Reactive Protein. Clin Chem 46(11) 1839-1842

Perez-Amodio S, Holownia P, Davey C, C (2001) Effects of the Ionic Environment, Charge, and Particle Surface Chemistry for Enhancing a Latex Homogeneous Immunoassay of C-Reactive Protein. Anal. Chem.73 (14) 3417–3425

Lucas L, Han J, Yoon J (2006) Using highly carboxylated microspheres to simplify immunoassays and enhance diffusional mixing in a microfluidic device. Colloids and Surf. B: Biointerfaces 49(2) 106-111

Fu L, Kang C, Zhang P, Kang X, Li X, Wang H, …Qiu Y (2015) A modified quick PETIA for detecting anti-CCP antibodies in human serum, Anal Methods Issue 7

Deegan O, Walshe K, Kavanagh K, Doyle S. (2003) Quantitative detection of C-reactive protein using phosphocholine-labelled enzyme or microspheres. Anal. Biochem. 312 p175–181 (turbidimetric assay, 0.21µm PS-COOH)

Koskinen JM, Soukka JM, Meltola NJ, & Koskinen JO. (2018). Microbial identification from faces and urine in one step by two-photon excitation assay technique. Journal of immunological methods, 460, 113-118.(Cat. PC05003, 3.2µm PS-COOH microspheres, coated with anti-Legionella or anti-adenovirus Ab in ArcDia’s TPX assay.)

Nanavaty N, Lin L, Hinckley SH, & Kuret J. (2017) Detection and quantification methods for fibrillar products of in vitro tau aggregation assays. In Tau Protein (pp. 101-111). Humana Press, New York, NY.(Anionic PS-COOH, 40-90nm as tau aggregation inducers)

PC0

Amine Polystyrene

Amine-functionalized microspheres offer further options for coupling of biomolecules. Glutaraldehyde is commonly used for the immobilization of amine-containing compounds, and specialized linkers are available for targeted coupling of thiol or carbohydrate moieties. Polymer microspheres are available as ~10% solids (w/w) aqueous suspensions in these standard amounts: 0.5g, 1.0g, 1.5g, and 5.0g. For microspheres larger than 1.00µm, please call for availability.

TechNote 205, Covalent Coupling
PDS 732, Polystyrene Microspheres

Catalog Number Nominal Diameter

Specification Range
PA02001 0.200µm 0.190 - 0.210µm
PA03001 0.500µm 0.470 - 0.530µm
PA03002 0.750µm 0.720 - 0.780µm
PA04001 1.0µm 0.95 - 1.05µm

References

Schleicher KD, Dettmer SL, Kapinos LE, Pagliara S, Keyser UF, Jeney S, LimRoderick YH (2014) Selective transport control on molecular velcro made from intrinsically disordered proteins. Nat Nano 9, 525-530

Bandura DR, Baranov VI, Ornatsky OI, Antonov A, Kinach R, Lou X… Tanner S.(2009) Mass Cytometry: Technique for Real Time Single Cell Multitarget Immunoassay Based on Inductively Coupled Plasma Time-of-Flight Mass Spectrometry. Anal Chem 81,6813–6822

PA0