Mouse Anti-Chicken MCAM-UNLB
Referência 8385-01
Tamanho : 0.5mg
Marca : Southern Biotech
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Telefone : +1 850 650 7790
| Clone | C264 |
|---|---|
| Isotype | Mouse (BALB/c) IgG2bκ |
| Isotype Control | Mouse IgG2b-UNLB (A-1) |
| Specificity | Chicken/Turkey MCAM |
| Alternative Names | CD146, HEMCAM, melanoma cell adhesion molecule |
| Description | Chicken MCAM, a member of the immunoglobulin superfamily of cell surface receptors, consists of the V-V-C2-C2-C2 Ig domains. It is a single chain glycoprotein with three mRNA splice variants. One has a short cytoplasmic tail, another has a long tail, and the third seems to lack transmembrane and cytoplasmic regions. It is highly homologous to gicerin, a molecule involved in neurite outgrowth and Wilm’s kidney tumor progression in the chicken, and to the human melanoma progression molecule MUC18. Chicken MCAM is expressed by c-kit+ hematopoietic progenitor cells in embryonic bone marrow, thymocytes, and capillary endothelial cells. MCAM-expressing cells sorted from bone marrow are enriched in progenitors capable of differentiating into T cells when transferred into the appropriate thymic microenvironment. MCAM has been proposed to be involved in cellular adhesion and homing processes. |
| Immunogen | Mixture of E15 and E16 thymocytes from H.B15, H.B19, and H.B21 chicken embryos |
| Conjugate | UNLB (Unconjugated) |
| Buffer Formulation | Borate buffered saline, pH 8.2 |
| Clonality | Monoclonal |
| Concentration | 0.5 mg/mL |
| Volume | 1.0 mL |
| Recommended Storage | 2-8°C |
| Applications | Flow Cytometry – Quality tested 1-8 Immunocytochemistry – Reported in literature 1 Immunoprecipitation – Reported in literature 1,2 |
| RRID Number | AB_2796533 |
| Gene ID | 448832 (Chicken) 100549798 (Turkey) |
| Gene ID Symbol | MCAM (Chicken) MCAM (Turkey) |
Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup
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- 1. Vainio O, Dunon D, Aïssi F, Dangy J, McNagny KM, Imhof BA. HEMCAM, an adhesion molecule expressed by c-kit+ hemopoietic progenitors. J Cell Biol. 1996;135:1655-68. (Immunogen, ICC, IP, FC)
- 2. Alais S, Allioli N, Pujades C, Duband J, Vainio O, Imhof BA, et al. HEMCAM/CD146 downregulates cell surface expression of β1 integrins. J Cell Sci. 2001;114:1847-59. (FC, IP)
- 3. Lampisuo M, Katevuo K, Lassila O. Antigenic phenotype of early intra-embryonic lymphoid progenitors in the chicken. Scand J Immunol. 1998;48:52-8. (FC)
- 4. Lampisuo M, Liippo J, Vainio O, McNagny KM, Kulmala J, Lassila O. Characterization of prethymic progenitors within the chicken embryo. Int Immunol. 1999;11:63-9. (FC)
- 5. Dunon D, Alliolo N, Vainio O, Ody C, Imhof BA. Quantification of T-cell progenitors during ontogeny: thymus colonization depends on blood delivery of progenitors. Blood. 1999;93:2234-43. (FC)
- 6. Guezguez B, Vigneron P, Alais S, Jaffredo T, Gavard J, Mège R, et al. A dileucine motif targets MCAM-l cell adhesion molecule to the basolateral membrane in MDCK cells. FEBS Lett. 2006;580:3649-56. (FC)
- 7. Guezguez B, Vigneron P, Lamerant N, Kieda C, Jaffredo T, Dunon D. Dual role of melanoma cell adhesion molecule (MCAM)/CD146 in lymphocyte endothelium interaction: MCAM/CD146 promotes rolling via microvilli induction in lymphocyte and is an endothelial adhesion receptor. J Immunol. 2007;179:6673-85. (FC)
- 8. Meyerhoff RR, Ali RA, Liu K, Huang G, Koci MD. Comprehensive analysis of commercially available mouse antichicken monoclonal antibodies for cross-reactivity with peripheral blood leukocytes from commercial turkeys. Poult Sci. 2012;91:383-92. (FC, Turkey Reactivity)