Images
Western Blot
Application

Western Blot

Western blot analysis of Lane 1: nonreduced (-) and Lane 2: reduced (+) mouse IgG2c with Mouse IgG2c monoclonal antibody, clone RM223 (Cat # MAB15110) under 0.5 ug/mL working concentration. This antibody reacts to nonreduced IgG2c.

Enzyme-linked Immunoabsorbent Assay
Application

Enzyme-linked Immunoabsorbent Assay

ELISA analysis of Mouse IgG2c monoclonal antibody, clone RM223 (Cat # MAB15110) at the following concentrations: 0.2 ug/mL, 0.1 ug/mL, 0.05 ug/mL, 0.025 ug/mL, 0.013 ug/mL, 0.006 ug/mL, 0.003 ug/mL. The plate was coated with 50 uL/well different amounts of mouse IgG2c. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

  • Specifications

    Product Description

    Rabbit recombinant monoclonal antibody raised against mouse IgG2c.Recombinant Antibody,Recombinant Antibodies,Recombinant Monoclonal Antibody,RecomAb,Recombinant Ab,Recombinant Monoclonal Antibodies,Recombinant Abs

    Antibody Species

    Rabbit

    Immunogen

    Original antibody is raised against mouse IgG2c.

    Sequence

    N/A

    Reactivity

    Mouse

    Specificity

    This antibody reacts to the Fc region of mouse IgG2c. No cross reactivity with mouse IgG1, IgG2a, IgG2b, IgG3, IgM, IgA, IgE, human IgG, or rat IgG.

    Form

    Liquid

    Purification

    Protein A affinity purification

    Isotype

    IgG

    Recommend Usage

    ELISA (0.01-0.2 ug/mL)
    Flow Cytometry
    Immunocytochemistry (0.5-2 ug/mL)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.5-2 ug/mL)
    Immunoprecipitation
    Western Blot (nonreduced only) (0.5-2 ug/mL)
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)

    Storage Instruction

    Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.

    Note

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

  • Applications

    Western Blot

    Western blot analysis of Lane 1: nonreduced (-) and Lane 2: reduced (+) mouse IgG2c with Mouse IgG2c monoclonal antibody, clone RM223 (Cat # MAB15110) under 0.5 ug/mL working concentration. This antibody reacts to nonreduced IgG2c.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

    Immunocytochemistry

    Immunoprecipitation

    Enzyme-linked Immunoabsorbent Assay

    ELISA analysis of Mouse IgG2c monoclonal antibody, clone RM223 (Cat # MAB15110) at the following concentrations: 0.2 ug/mL, 0.1 ug/mL, 0.05 ug/mL, 0.025 ug/mL, 0.013 ug/mL, 0.006 ug/mL, 0.003 ug/mL. The plate was coated with 50 uL/well different amounts of mouse IgG2c. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

    Flow Cytometry

  • Gene Info — Igh-1b

    Entrez GeneID

    404711

    Gene Name

    Igh-1b

    Gene Alias

    IGHG2C

    Gene Description

    immunoglobulin heavy chain 1b (serum IgG2c)

    Gene Ontology

    Hyperlink

    Other Designations

    -