K1307-5ml | mRNA Magnetic Purification Kit Clinisciences

Description

mRNA Magnetic Purification Kit is designed for purifying mRNA from total RNA or in vitro transcription, which uses monodisperse superparamagnetic beads that covalently couple oligomeric dT sequences on its surface. High purity mRNA is achieved by base pairing between the oligo-DT sequence and the poly(A) tail of the mRNA. The dT sequence combined with mRNA can be directly used as the first chain reaction primer to synthesize cDNA, or mRNA can be eluted from the beads for various downstream molecular experiments, such as RT-PCR, RPA (ribonuclease protection assay), library construction, Northern Blot analysis, next-generation sequencing, etc.

Quality Control

Protocol

Components and Storage

Components	2 mL	5 mL
Oligo (dT) 25 beads (10 mg/mL)	2*1 mL	5*1 mL
Binding Buffer	5 mL	3*5 mL
Washing Buffer	5 mL	3*5 mL
Tris-HCl Buffer	1 mL	3*1 mL
RNase-free water	2*1 mL	5*1 mL
Store at 4 °C, no freezing!

mRNA Magnetic Purification Kit

Referência K1307-5ml

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Marca : APExBIO Technology

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Description

Quality Control

Components and Storage