Mycoplasma pneumoniae IgM ELISA Kit

Referência EA101078

Tamanho : 1kit

Solicitar mais informações

Contactar o distribuidor local :


Telefone :

Mycoplasma pneumoniae IgM ELISA Kit

SKU
EA101078
Mycoplasma pneumoniae IgM ELISA Kit
Specifications
Product Data
Assay Length 3 hours
Assay Type Solid Phase Sandwich ELISA
Assay Type Sandwich
Background The Mycoplasma pneumoniae IgM ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA). Specimen samples are diluted with Sample Diluent and additionally incubated with IgG-RF-Sorbent, containing hyper-immune anti-human IgG-class antibody to eliminate competitive inhibition from specific IgG and to remove rheumatoid factors. This pretreatment avoids false results. Microtiter wells as a solid phase are coated with Mycoplasma pneumoniae antigen. Pretreated sample specimens and ready-for-use controls are pipetted into these wells. During incubation Mycoplasma pneumoniae-specific antibodies of high specimens and controls are bound to the immobilized antigens. After a washing step to remove unbound sample and control material horseradish peroxidase conjugated anti-human IgM antibodies are dispensed into the wells. During a second incubation this anti IgM conjugate binds specifically to IgM antibodies resulting in the formation of enzyme-linked immune complexes. After a second washing step to remove unbound conjugate the immune complexes formed (in case of high results) are detected by incubation with TMB substrate and development of a blue color. The blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid. The intensity of this color is directly proportional to the amount of Mycoplasma pneumoniae-specific IgM antibody in the sample specimen. Absorbance at 450 nm is read using an ELISA microtiter plate reader.
Components See the product information for more details
Format 96-well strip plate
Interference No significant interference observed with available related molecules.
Reactivity Human
Reactivity Human
Reactivity Human
Sample Type Cell culture supernatant, serum, plasma
Sample Volume 10 ul
Signal Colorimetric
Species Human
Specificity Natural and recombinant human Mycoplasma pneumoniae IgM
New No
Description Mycoplasma pneumoniae IgM ELISA Kit

Você também pode estar interessado nos seguintes produtos:



Referência
Descrição
Cond.
Price Bef. VAT