Oct4 (POU5F1) (NM_203289) Human Recombinant Protein
Referência TP760938
Tamanho : 50ug
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Oct4 (POU5F1) (NM_203289) Human Recombinant Protein
SKU
TP760938
Purified recombinant protein of Human POU class 5 homeobox 1 (POU5F1/OCT4), transcript variant 2, full length, with N-terminal HIS tag, expressed in E. coli, 50ug
In Stock*
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | E. coli |
| Expression cDNA Clone or AA Sequence | Protein Sequence A DNA sequence encoding human full-length POU5F1/OCT4 |
| Tag | N-His |
| Predicted MW | 21.1 kDa |
| Concentration | >0.05 µg/µL as determined by microplate BCA method |
| Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| Buffer | 25 mM Tris-HCl, pH 8.0, 150 mM NaCl, 1% sarkosyl, 10% glycerol |
| Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
| Storage | Store at -80°C. |
| Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Reference Data | |
| RefSeq | NP_976034 |
| Locus ID | 5460 |
| UniProt ID | M1S623 |
| Cytogenetics | 6p21.33 |
| RefSeq Size | 1155 |
| RefSeq ORF | 795 |
| Synonyms | Oct-3; Oct-4; OCT3; OCT4; OTF-3; OTF3; OTF4 |
| Summary | This gene encodes a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency. Aberrant expression of this gene in adult tissues is associated with tumorigenesis. This gene can participate in a translocation with the Ewing's sarcoma gene on chromosome 21, which also leads to tumor formation. Alternative splicing, as well as usage of alternative AUG and non-AUG translation initiation codons, results in multiple isoforms. One of the AUG start codons is polymorphic in human populations. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq, Oct 2013] |
| Protein Families | Adult stem cells, Cancer stem cells, Embryonic stem cells, Induced pluripotent stem cells, Stem cell - Pluripotency, Transcription Factors |
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Recombinant Protein Resources |
| SKU | Description | Size | ||
|---|---|---|---|---|
| PH311998 | POU5F1 MS Standard C13 and N15-labeled recombinant protein (NP_002692) | 10 ug | | |
| LC400950 | POU5F1 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug | | |
| LC404373 | POU5F1 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug | | |
| LY400950 | Transient overexpression lysate of POU class 5 homeobox 1 (POU5F1), transcript variant 1 | 100 ug | | |
| LY404373 | Transient overexpression lysate of POU class 5 homeobox 1 (POU5F1), transcript variant 2 | 100 ug | | |
| TP311998 | Recombinant protein of human POU class 5 homeobox 1 (POU5F1), transcript variant 1, 20 µg | 20 ug | | |
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.