PD-L1 antibody [29E.2A3] (PerCP-Cy5.5)
Referência GTX01495-11-100test
Tamanho : 100test
Marca : GeneTex
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![Anti-PD-L1 antibody [29E.2A3] (PerCP-Cy5.5) used in Flow cytometry (FACS). GTX01495-11](http://www.genetex.com/templates/default/js/lightbox/images/loading.gif "Anti-PD-L1 antibody [29E.2A3] (PerCP-Cy5.5) used in Flow cytometry (FACS). GTX01495-11")
Host | Mouse |
|---|---|
Clonality | Monoclonal |
Clone Name | 29E.2A3 |
Isotype | IgG2b |
Application | FACS |
Reactivity | Human |
| Package | 100 test |
Datasheet File View product citations for antibody GTX01495-11 on CiteAb
PRODUCT
Summary
The 29E.2A3 antibody reacts with human CD274 which is also known as PD-L1 or B7-H1. CD274 is a type 1 transmembrane glycoprotein and a member of the Ig superfamily. PD-L1 is a ligand for CD279 (PD-1) and plays an important role in regulating immune cell responses. CD274 is expressed on activated monocytes, macrophages, dendritic cells, and T cells.
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application | Recommended Dilution |
|---|---|
| FACS | 1 μg (5 μl) for 10⁵-10⁸ cells in 100 μl sample per test |
Not tested in other applications.
Calculated MW
33 kDa. ( Note )
PROPERTIES
Form
Liquid
Buffer
10mM NaH₂PO₄, 150mM NaCl, 0.1% Gelatin
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Purification
Purified by affinity chromatography
From tissue culture supernatant
From tissue culture supernatant
Conjugation
Peridinin-chlorophyll proteins-Cyanine5.5 (PerCP-Cy5.5) Wavelength
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
CD274 molecule , B7H , B7H1 , CD274 , PDCD1L1 , PDCD1LG1 , PDL1 , PD-L1
Cellular Localization
Cell membrane,Endomembrane system
Background
This gene encodes an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
Database
DATA IMAGES
| | GTX01495-11 FACS Image FACS analysis of human PBMCs (PHA stimulation for 3 days) using GTX01495-11 PD-L1 antibody [29E.2A3] (PerCP-Cy5.5). |
Clone Reference
Chen JY et al. Br J Cancer 2021; 124 (2):484-493 Lysine demethylase 2A expression in cancer-associated fibroblasts promotes breast tumour growth.
2019; TLR9 Mediated Tumor-Stroma Interactions in Human Papilloma Virus (HPV)-Positive Head and Neck Squamous Cell Carcinoma Up-Regulate PD-L1 and PD-L2.
2014; A mechanism of hypoxia-mediated escape from adaptive immunity in cancer cells.
2013; Allogenicity of human cardiac stem/progenitor cells orchestrated by programmed death ligand 1.
2003; Blockade of programmed death-1 ligands on dendritic cells enhances T cell activation and cytokine production.
Submit a Reference REVIEW
Clone Reference
Chen JY et al. Br J Cancer 2021; 124 (2):484-493 Lysine demethylase 2A expression in cancer-associated fibroblasts promotes breast tumour growth.
2019; TLR9 Mediated Tumor-Stroma Interactions in Human Papilloma Virus (HPV)-Positive Head and Neck Squamous Cell Carcinoma Up-Regulate PD-L1 and PD-L2.
2014; A mechanism of hypoxia-mediated escape from adaptive immunity in cancer cells.
2013; Allogenicity of human cardiac stem/progenitor cells orchestrated by programmed death ligand 1.
2003; Blockade of programmed death-1 ligands on dendritic cells enhances T cell activation and cytokine production.
2001; PD-L2 is a second ligand for PD-1 and inhibits T cell activation.
| SDS | |
|---|---|
| Sodium Azide.pdf | |