Application
| WB, IHC |
---|---|
Primary Accession | Q9Y6VO |
Other Accession | NP_055325.2 |
Host | Rabbit |
Reactivity | Human, Mouse, Rat |
Clonality | Polyclonal |
Description | Rabbit Anti-Human Piccolo Polyclonal |
Target/Specificity | Detects ~550kDa. No cross-reactivity to Bassoon. |
Other Names | ACZ Antibody, Aczonin Antibody, Brain-derived HLMN protein Antibody, DKFZp779G1236 Antibody, KIAA0559 Antibody, Multidomain presynaptic cytomatrix protein Antibody, PCLO Antibody, PCLO_HUMAN Antibody, Piccolo (presynaptic cytomatrix protein) Antibody, Piccolo mouse homolog of Pico Antibody, Presynaptic cytomatrix protein Antibody, Protein piccolo Antibody |
Immunogen | Full length protein, BC001304.1 |
Purification | Protein A Purified |
Storage | -20ºC |
Storage Buffer | PBS pH7.4, 50% glycerol, 0.09% sodium azide |
Shipping Temperature | Blue Ice or 4ºC |
Certificate of Analysis | 1 µg/ml of SPC-197 was sufficient for detection of Piccolo in 10 µg of rat brain tissue lysate by colorimetric immunoblot analysis using goat ant rabbit IgG:HRP as the secondary antibody. |
Cellular Localization | Cell Junction | Synapse |
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Provided below are standard protocols that you may find useful for product applications.
Background
Piccolo, also referred to as Aczonin, is a large protein which consists of an N-terminal Zn2+ finger, several piccolo-bassoon homology domains and C-terminal PDZ and C2 domains (1, 2). In general it is found together with bassoon, a related huge multi-domain protein of the CAZ (cytoskeletal matric assembled at active zones) (3). Piccolo is a scaffolding protein for proteins involved in endo- and exocytosis of synaptic vesicles. Piccolo has also been shown to interfere with clathrin medicated endocytosis by binding to the F-actin and dynamin binding protein Abp1 (2).
References
1. Gerber S.H., Garcia J., Rizo J., Sudhof T.C. (2001) EMBO J. 20(7): 1605-1619.
2. Wang X., et al. (1999) J Cell Biol. 147(1): 151-162.
3. Dresbach T., et al. (2006) J Biol Chem. 281(9):6038-6047.