Specifications
Product Data
C-Tag	mGFP
E. coli Selection	Chloramphenicol (34 ug/mL)
Features	This Lenti vector is a third generation of lentiviral vector. All OriGene’s TrueORF clone can be shuttled into this vector via PrecisionShuttling, a simple cut-and-ligation process. The ORF cloned in this vector can be expressed as a tagged protein with a c-terminal monomeric GFP tag. mGFP (scientific name mTagGFP) is the improved variant of tagGFP, the mutant of the Aequorea macrodactyla GFP-like protein. mGFP possesses bright green fluorescence with excitation/emission maxima at 483 and 506 nm, respectively. Compared to EGFP, mGFP provides about the same brightness of fluorescence but is significantly more pH stable. mGFP is specially optimized for expression at 37°C. Because of monomeric nature, mGFP is mainly intended for protein localization studies and expression in long-term cell cultures. In FRET applications, mGFP can be used as a donor for red fluorescent protein mRFP or as an acceptor for blue fluorescent protein mBFP. A brief lentiviral packaging protocol is provided. V2_F Primer can be used to sequence the target sequence after cloning. mGFP_R Primer can be used to sequence the target sequence after cloning.
Schematic of the multiple cloning sites

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Documents

Product Manuals
TrueORF and PrecisionShuttle cloning vectors Application Guide
FAQs
FAQ for Tagged Cloning Vectors
SDS for PS100071

Resources

Vector Resources
Video Tutorial: Clone Selection Guide Product Video: Why Should You Choose TrueORF GOLD shRNA Selection Guide Protocol: Lentivirus packaging CRISPR Knockout kit manual CRISPR vector manual CRISPR Trangene Knockin at AAVS1 and ROSA26 loci manual CRISPR activation (CRISPRa) and CRISPR interference (CRISPRi) manual AAV Biosafety

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RC218134L2

SIRT1 (NM_012238) Human Tagged Lenti ORF Clone

10ug

GE100111	Forward primer to sequence targets cloned in expression vectors.	1 nmol
GE100125	Reverse primer to sequence targets cloned in expression vectors.	1 nmol
TR30037	Lenti-vpak packaging kit - packaging plasmids and transfection reagent	10 reactions
TF81001	TurboFectin Transfection Reagent (1 mL in 1 vial)	1 ml in 1 vial

pLenti-C-mGFP Lentiviral Gene Expression Vector

Referência PS100071

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pLenti-C-mGFP Lentiviral Gene Expression Vector

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