PLGLA ELISA Kit (Human)

Referência OKWB00184

Tamanho : 96Wells

Marca : Aviva Systems Biology

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Datasheets/ManualsPrintable datasheet for PLGLA ELISA Kit (Human) (OKWB00184)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationELISA-Sandwich
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit Linearity
Sample Type1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)85-100%88-104%87-95%95-104%
EDTA Plasma (n=5)83-100%87-100%82-98%83-100%
Heparin Plasma (n=5)88-99%86-98%82-99%85-100%
ELISA Kit PrincipleAviva Systems Biology PLGLA ELISA Kit (Human) (OKWB00184) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PLGLA has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PLGLA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PLGLA captured in the well.
ELISA Kit Range0.313-20ng/ml
ELISA Kit Recovery
Sample TypeRangeMean
Serum (n=5)85-10593
EDTA Plasma (n=5)88-10393
Heparin Plasma (n=5)88-10495
ELISA Kit ReproducibilityMean Intra-Assay Precision <10%
Mean Inter-Assay Precision <12%
ELISA Kit Component
ComponentAmount
PLGLA Microplate96 Wells (12 x 8 Well strips)
PLGLA Lyophilized Standard2 x 20 ng
100X PLGLA Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 20 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
Sensitivity0.188 ng/mL
Assay InfoAssay Type: Quantitative Sandwich ELISA
Gene SymbolPLGLA
Alias SymbolsPlasminogen-like protein A1;Plasminogen-related protein A.
Protein NamePlasminogen-like protein A
Description of TargetMay bind non-covalently to lysine binding sites present in the kringle structures of plasminogen. This may interfere with the binding of fibrin or alpha-2-antiplasmin to plasminogen and may result in the localization of activity at sites necessary for extracellular matrix destruction (By similarity).
Uniprot IDQ15195

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