Precast GelRed® Agarose Gels, 1% Agarose/TAE
Referência 41041
Tamanho : packof10
Marca : Biotium
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Telefone : +1 850 650 7790
Precast GelRed® Agarose Gels, 1% Agarose/TAE
Ready-to-use 1% agarose/TAE gels precast with GelRed® Nucleic Acid Gel Stain. The gels are suitable for visualizing 250-12,000 bp DNA fragments.
Technology
Nucleic Acid Gel Stains
Protocols
SDS
Supporting Documents
Product Description
Precast GelRed® Agarose Gels are ready-to-use gels intended for DNA gel electrophoresis. The 1% agarose gels were cast in TAE buffer and contain highly sensitive GelRed® Nucleic Acid Gel Stain, saving a significant amount of time by eliminating the need for casting gels.
- Ready-to-use 1% agarose/TAE gels precast with GelRed®
- Saves time by eliminating the need for casting gels
- Ultra-sensitive GelRed® for brighter DNA staining than EtBr
- GelRed® is a non-mutagenic, non-toxic replacement for hazardous EtBr
- Replaces EtBr with no change to equipment or optical settings
- Suitable for visualizing 250-12,000 bp DNA fragments
- Gels available with 8 or 18 wells and sold in packs of 10 gels
Biotium offers 1X TAE (1 L) Buffer Powder Packets (Cat. No. 22031) that are suitable for use with Precast GelRed® Agarose TAE Gels.
GelRed®: A Superior Replacement for EtBr
GelRed® and ethidium bromide (EtBr) have very similar excitation and emission wavelengths, so you can directly replace EtBr with GelRed® without changing your existing imaging system. In addition, GelRed® is far more sensitive than EtBr. Staining nucleic acids with GelRed® is compatible with downstream applications such as sequencing and cloning. GelRed® can be removed from DNA using a gel extraction kit, or by phenol/chloroform extraction followed by ethanol precipitation. To learn more, learn more about GelRed® or read our GelRed® FAQs.
Non-Mutagenic and Safer for the Environment
GelRed® was subjected to a series of tests at Biotium and by three independent testing services to assess the dye’s safety for routine handling and disposal. Test results confirm that the dye is impenetrable to both latex gloves and cell membranes. The dye is non-cytotoxic and non-mutagenic at concentrations well above the working concentrations used in gel staining. GelRed® successfully passed environmental safety tests in compliance with CCR Title 22 Hazardous Waste Characterization, under which GelRed® is classified as non-hazardous waste. See the GelRed® and GelGreen® Safety Report.
See the table below for a detailed comparison of available GelRed® and GelGreen® formats.
Precast GelRed® Agarose Gels, 1% Agarose/TAE
| Catalog No. | # Wells | Gel Dimensions (L x W) | Well Dimensions (L x W) | Load Volume | Size |
|---|---|---|---|---|---|
| 41041 | 8 | 60 x 56 mm | 4.4 x 1 mm | 22 uL per well | 10 gels |
| 41042 | 18 | 60 x 116 mm | 4.4 x 1 mm | 22 uL per well | 10 gels |
Choose the Right Stain for Your Application
| Product / Method | Procedure | Advantages | Disadvantages | Recommended for |
|---|---|---|---|---|
| DNA staining with EMBER™ Ultra DNA Gel Kit | Agarose is supplied pre-coated with EMBER™ Ultra Dye, just dissolve, heat, and pour. | • Safer and more convenient, no need to handle concentrated dye • Superior sensitivity, detect as little as ≤1 ng DNA • No need for post-electrophoresis staining • Optimal for blue LED gel imagers | • Not suitable for PAGE, DGGE, EMSA, or PFGE gels • Dye may cause band migration issues when loading larger amounts of DNA (more than ~200 ng/band), or for some restriction digests | • Routine agarose gels |
| RNA staining with EMBER™ Ultra RNA Gel Kit | Agarose is supplied pre-coated with EMBER™ Ultra Dye, just dissolve, heat, and pour. | • Safer and more convenient stain for RNA, no need to handle concentrated dye • Superior sensitivity, detect as little as ≤5 ng RNA • No need for post-electrophoresis staining • Included loading dye contains formamide for denaturing • Optimal for blue LED gel imagers | • Will stain DNA as well as RNA • Dye may cause band migration issues when loading larger amounts of RNA (more than ~200 ng/band) | • Routine RNA gel electrophoresis • Evaluate total RNA integrity and DNA contamination |
| DNA staining with Precast GelRed® Agarose Gels | Load samples and run the precast gel that is prestained with GelRed® | • Saves time by eliminating the need for casting gels • Safer handling by eliminating the need to handle concentrated dye | • Gels are precast in 1% agarose and are only suitable for visualizing 250-12,000 bp DNA fragments • Gels have a fixed amount of 8 wells per gel • Not suitable for PAGE, DGGE, EMSA, or PFGE gels • Dye may cause band migration issues when loading larger amounts of DNA (more than ~100 ng/band), or for some restriction digests | • Routine agarose gels • Recommended loading 50-200 ng ladder or 2-5 uL PCR product ( ~100 ng/band or less) |
| DNA prestaining with GelRed® Prestain Plus 6X DNA Loading Dye | GelRed® loading buffer is added directly to the DNA sample before loading | • Fast & simple: one-step sample loading & DNA staining • Less concentrated dye for safer handling • Can re-run a gel to use empty lanes | • Not recommended for PAGE, DGGE, EMSA, or PFGE gels • Dye may cause band migration issues when loading larger amounts of DNA (more than ~100 ng/band), or for some restriction digests | • Routine agarose gels • Recommended loading 50-200 ng ladder or 2-5 uL PCR product ( ~100 ng/band or less) |
| Precast staining with GelRed® 10,000X in water or GelGreen® 10,000X in water | GelRed® or GelGreen® is mixed with molten agarose before gel casting | Familiar protocol, rapid results | ||
| Precast staining with GelRed® Agarose LE or GelGreen® Agarose LE | Agarose is supplied pre-coated with GelRed® or GelGreen®, just dissolve, heat, and pour | Safer & more convenient, no need to handle concentrated dye | ||
| Post-electrophoresis staining with GelRed® 10,000X in water or GelGreen® 10,000X in water - or - GelRed® 3X in water | No fluorescent dye is added to the gel, it is stained in 3X GelRed® or 3X GelGreen® solution after electrophoresis | • Most accurate sizing/sharpest bands • Staining solution can be re-used • Enhance sensitivity by adding NaCl | Extra staining step (up to 30 minutes) after electrophoresis (some customers report good results after only 5 minutes if dye is not reused) | • Highly accurate band sizing • Gels with more than ~100 ng DNA per band • Analyzing restriction digests |
| Post-electrophoresis staining of PAGE gels using PAGE GelRed® 10,000X or 1X in water | No fluorescent dye is added to the gel, it is stained in 1X PAGE GelRed® solution after electrophoresis | • Formulated for efficient penetration and staining of polyacrylamide gels • Like the classic GelRed®, it is safe and environmentally friendly | Extra staining step of approx. 30 minutes after electrophoresis | Staining of nucleic acids in PAGE gels |
References
Download a list of selected References for GelRed® and GelGreen®.