These antibody-free human primary cells were initiated from normal human coronary artery without the use of positive selection of antibodies.
Primary Human Coronary Artery Endothelial Cells (ACBRI 377)
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- PRODUCT INFO
- CITATIONS
- TESTS
- DOCUMENTATION
Increase Biological Relevance with Human Primary Cells
Our antibody-free primary cells can offer a more biologically relevant cell culture tool for scientists to enhance their research insights. These primary cells were originated using Cell Systems Complete Serum-Free Medium (SF-4Z0-500) and subsequently grown and passaged in Cell Systems Complete Classic Medium (4Z0-500). The cells are cryopreserved in Cell Systems Cell Freezing Medium (4Z0-705).
- Isolated from normal, healthy donor tissue
- Available at Passage 3 (<12 cumulative population doublings)
- Each vial contains approximately 1 x 106 cells
- Each vial will initiate a Passage 4 cell culture in a 75 cm2 flask
- Available in reserved lots to enhance consistency in your research program
Each vial of cells is shipped to customers with 1mL of Bac-Off® and .25mL of CultureBoost™.
A Selection of Citations for ACBRI 377 from Scientific Journals
Discover additional research on Google Scholar that utilizes Cell Systems primary cells.
- "Differential procoagulatory response of microvascular, arterial and venous endothelial cells upon inflammation in vitro" Brandtner et al. Thrombosis Research, 2021.
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"RAGE and CCR7 mediate the transmigration of Zika-infected monocytes through the blood-brain barrier" Costa de Carvalho et al. Immunobiology, 2019.
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"Expression Profiling of the Ephrin (EFN) and Eph Receptor (EPH) Family of Genes in Atherosclerosis-Related Human Cells" Saamoto et al. J International Medical Research, 2011.
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"Endothelial microparticles induce formation of plateletaggregates via a von Willebrand factor/ristocetin dependentpathway, rendering them resistant to dissociation" Jimenez et al. Journal of Thrombosis and Haemostasis, 2005.
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"Role of ceramide in activation of stress-associated MAP kinases by minimally modified LDL in vascular smooth muscle cells" Elsevier, 2004.
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"Endothelial cells release phenotypically and quantitatively distinct microparticles in activation and apoptosis" Jimenez et al. Thrombosis Research, 2003.
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"Enhancement of sphingosine 1-phosphate-induced migration of vascular endothelial cells and smooth muscle cells by an EDG-5 antagonist" Osada et al. Elsevier, 2002.
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"P-285: Insulin enhances bradykinin -stimulated nitric oxide production in vascular endothelial cells" Matsumoto et al. American Journal of Hypertension, 2002.
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"P-202: Eicosapentaenoic acid (EPA) prevents vascular endothelial cell death induced by high glucose" Yamamoto et al. American Journal of Hypertension, 2002.
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"Identification of macrophage migration inhibitory factor as a potent endothelial cell growth-promoting agent released by ectopic human endometrial cells" Yang et al. The Journal of Clinical Endocrinology & Metabolism, 2000.
Standard Tests
| TESTS | RESULTS |
| HIV Serologic Test (donor level HIV AB EIA) | Negative |
| HIV PCR Test (frozen cell pool by CLIA Licensed Clinical Lab) | Negative |
| Hepatitis B (HBV) and Hepatitis C (HCV) PCR Test (at frozen cell level) | Negative |
Retail Production (P3)Tests
| TESTS | RESULTS |
| Bacterial Sterility (culture method) by independent lab | Pass |
| Fungal Sterility (culture method) by independent lab | Pass |
| Mycoplasma Sterility (culture method) by independent lab | Pass |
Cell Markers and Functional Tests
| TESTS | RESULT |
| Inducible expression of CD 62E (E-Selectin) | > 90% positive by immunofluorescence |
| Cytoplasmic VWF / Factor VIII | > 95% positive by immunofluorescence |
| Cytoplasmic uptake of Di-I-Ac-LDL | > 95% positive by immunofluorescence |
