Protein A/G PLUS-Agarose
Referência sc-2003
Tamanho : 2ml
Marca : Santa Cruz Biotechnology
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Protein A/G PLUS-Agarose: sc-2003
- Permet une liaison pour toutes les espèces d'anticorps et sous-classes d'IgG reconnues par la protéine A ou la protéine G
- fourni en 0.5 ml agarose/2.0 ml; 100 réactions
- volume recommandé de 20 µl par réaction d'mmunoprécipitation
- la capacité de liaison est de 10 mg d'anticorps par 1 ml de billes tassées, décantées et non centrifugées
- Pre-traité pour réduire les liaisons immunoglobulines non-specifiques
- couplé à de la Sepharose CL-6B
- liaison forte aux IgG1, IgG2a, IgG2b et IgG3 de souris, aux IgG2a et IgG2c de rat, aux IgG1, IgG2, IgG3 et IgG4 humaines, aux IgG de lapin, de chèvre, bovines, porcines, ovines, canines, félines, et équines
- liaison moyenne aux IgG1 et IgG2b de rat et humaines IgM, IgA et IgE.
- pré-lavé et prêt à l'emploi
ACCÈS RAPIDE AUX LIENS
and Cruz Marker™ MW Standards Promotional
Molecular purification methods such as immunoprecipitation (IP), co-IP and chromatin IP are important tools in studying lowly expressed proteins, protein-protein interactions or protein-nucleic acid interactions. Santa Cruz Biotechnology offers a comprehensive array of research support products for purification techniques. Protein A/G PLUS is an essential specialty reagent used for isolating immunoglobulins from nearly any mammalian species. Protein A/G PLUS is a genetically-engineered protein that combines binding profiles of Protein A and Protein G, thereby producing an exceptionally high-capacity reagent. Protein A/G PLUS works through non-covalent high affinity binding of either protein A or protein G to the Fc regions of mammalian antibody isotypes, specifically IgG, IgA, IgE and IgM. Protein A/G PLUS is a powerful reagent for purification and detection of mouse monoclonal antibodies from IgG subclasses without interference from other serum proteins. Protein A/G PLUS is linked to a high-quality Agarose resin, CL-6B, which makes it a superior and premium product that produces high purity and purification yield of IgGs from mammalian samples.
References:
- Tyrosine phosphorylation of Shc proteins in normal CD34+ progenitor cells and leukemic cells. | Yang, J., et al. 1999. Blood. 94: 373-4. PMID: 10428548
- Silibinin causes cell cycle arrest and apoptosis in human bladder transitional cell carcinoma cells by regulating CDKI-CDK-cyclin cascade, and caspase 3 and PARP cleavages. | Tyagi, A., et al. 2004. Carcinogenesis. 25: 1711-20. PMID: 15117815
- Mechanisms involved in resveratrol-induced apoptosis and cell cycle arrest in prostate cancer-derived cell lines. | Benitez, DA., et al. 2007. J Androl. 28: 282-93. PMID: 17050787
- DNA damage induced by multiwalled carbon nanotubes in mouse embryonic stem cells. | Zhu, L., et al. 2007. Nano Lett. 7: 3592-7. PMID: 18044946
- Antigen-subtracted 2-DE/MS strategy, a novel proteomic analysis platform. | Zhao, P., et al. 2011. Arch Toxicol. 85: 35-41. PMID: 20407759
- Determination of histone acetylation status by chromatin immunoprecipitation. | Galdieri, L., et al. 2012. Methods Mol Biol. 809: 255-65. PMID: 22113281
- Generation and characterization of polyclonal antibodies against mouse T-cell immunoglobulin and immunoreceptor tyrosine-based inhibitory domain by DNA-based immunization. | Gao, Y., et al. 2014. Transplant Proc. 46: 260-5. PMID: 24507063
- Proteomic analysis of Fasciola hepatica excretory and secretory products (FhESPs) involved in interacting with host PBMCs and cytokines by shotgun LC-MS/MS. | Liu, Q., et al. 2017. Parasitol Res. 116: 627-635. PMID: 27866265
- Characterization of a Protein Interactome by Co-Immunoprecipitation and Shotgun Mass Spectrometry. | Maccarrone, G., et al. 2017. Methods Mol Biol. 1546: 223-234. PMID: 27896772
- The EMSY threonine 207 phospho-site is required for EMSYdriven suppression of DNA damage repair. | Jelinic, P., et al. 2017. Oncotarget. 8: 13792-13804. PMID: 28099152
- Biomarker analysis of fucosylated kininogen through depletion of lectin reactive heterophilic antibodies in hepatocellular carcinoma. | Wang, M., et al. 2018. J Immunol Methods. 462: 59-64. PMID: 30144410
- Analysis of PD-L1 Transcriptional Regulation in Ovarian Cancer Cells by Chromatin Immunoprecipitation. | Zou, Y., et al. 2020. Methods Mol Biol. 2108: 229-239. PMID: 31939185
- Genistein suppresses ox-LDL-elicited oxidative stress and senescence in HUVECs through the SIRT1-p66shc-Foxo3a pathways. | Zhang, H., et al. 2022. J Biochem Mol Toxicol. 36: e22939. PMID: 34719845
- Inhibitors of p38 mitogen-activated protein kinase promote neuronal survival in vitro. | Horstmann, S., et al. 1998. J Neurosci Res. 52: 483-90. PMID: 9589393
Informations pour la commande
Nom du produit | Ref. Catalogue | COND. | Prix HT | QTÉ | Favoris | |
Protein A/G PLUS-Agarose | sc-2003 | 2 ml | .00 |