Pvr (NM_027514) Mouse Tagged ORF Clone

Referência MR206417

Tamanho : 10ug

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Pvr (NM_027514) Mouse Tagged ORF Clone

SKU
MR206417
Pvr (Myc-DDK-tagged) - Mouse poliovirus receptor (Pvr)

Expression-ready ORF plasmid with C-terminal tag(s)

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Bulk Requests & Clone Modifications
Specifications
Product Data
Target Symbol Pvr
Synonyms 3830421F03Rik; CD155; D7Ertd458e; HVED; mE4; necl-5; PVS; Taa1; Tage4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR206417 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTCAACTCGCCCGAGCCACCCGCTCCCCGCTGTCATGGCTGCTGCTGCTGTTCTGCTATGCACTCC
GGAAAGCGGGTGGGGATATACGTGTGCTGGTGCCCTACAATTCGACAGGCGTCTTGGGAGGGTCGACCAC
CTTGCACTGTAGTCTGACTTCTAATGAGAATGTGACTATCACTCAAATAACCTGGATGAAGAAGGATTCA
GGTGGATCCCACGCTCTTGTGGCTGTCTTCCACCCCAAGAAGGGGCCCAACATCAAAGAGCCAGAGAGGG
TGAAATTCTTGGCTGCCCAACAGGATCTGAGGAACGCATCTCTGGCCATCTCGAACTTAAGTGTAGAAGA
CGAAGGCATCTATGAATGTCAGATTGCCACATTCCCCAGAGGCAGTAGAAGCACCAATGCCTGGCTGAAG
GTGCAAGCCCGACCTAAGAACACTGCAGAGGCCCTGGAGCCCTCTCCCACCTTGATACTGCAGGATGTGG
CTAAATGCATCTCTGCCAATGGTCACCCTCCTGGACGAATCTCTTGGCCCTCGAATGTGAATGGAAGTCA
CCGTGAAATGAAGGAACCAGGGTCCCAGCCGGGCACCACCACAGTTACCAGCTACCTCTCCATGGTACCT
TCTCGCCAGGCAGACGGCAAGAACATCACCTGCACGGTGGAGCATGAAAGCTTACAGGAGCTGGACCAGC
TGCTGGTGACCCTTTCCCAACCCTATCCACCTGAAAACGTGTCCATCTCTGGCTATGACGGCAACTGGTA
TGTTGGCCTCACTAACTTGACCCTGACCTGTGAAGCTCACAGCAAACCAGCGCCTGACATGGCTGGATAT
AACTGGAGCACGACCACGGGTGACTTTCCCAACTCTGTTAAGCGCCAGGGCAATATGCTTCTAATCTCCA
CCGTAGAGGATGGTCTCAATAACACGGTCATTGTGTGCGAAGTCACCAATGCCCTAGGGTCTGGGCAGGG
CCAAGTGCACATCATTGTTAAAGAGAAACCTGAGAATATGCAGCAAAATACAAGATTACACCTAGGCTAC
ATCTTTCTTATCGTCTTTGTCCTCGCTGTAGTCATCATCATCGCAGCACTATACACTATACGAAGATGCA
GGCATGGTCGTGCTCTGCAGTCCAATCCCTCAGAGAGGGAGAACGTCCAGTATTCATCTGTGAACGGCGA
CTGTAGACTGAACATGGAGCCAAACAGCACAAGG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR206417 protein sequence
Red=Cloning site Green=Tags(s)

MAQLARATRSPLSWLLLLFCYALRKAGGDIRVLVPYNSTGVLGGSTTLHCSLTSNENVTITQITWMKKDS
GGSHALVAVFHPKKGPNIKEPERVKFLAAQQDLRNASLAISNLSVEDEGIYECQIATFPRGSRSTNAWLK
VQARPKNTAEALEPSPTLILQDVAKCISANGHPPGRISWPSNVNGSHREMKEPGSQPGTTTVTSYLSMVP
SRQADGKNITCTVEHESLQELDQLLVTLSQPYPPENVSISGYDGNWYVGLTNLTLTCEAHSKPAPDMAGY
NWSTTTGDFPNSVKRQGNMLLISTVEDGLNNTVIVCEVTNALGSGQGQVHIIVKEKPENMQQNTRLHLGY
IFLIVFVLAVVIIIAALYTIRRCRHGRALQSNPSERENVQYSSVNGDCRLNMEPNSTR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN NM_027514
ORF Size 1224 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_027514.1, NM_027514.2, NP_081790.1
RefSeq Size 2902 bp
RefSeq ORF 1227 bp
Locus ID 52118
Cytogenetics 7 9.95 cM
MW 44.7 kDa

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2040-08
 1.0mg