ASM10544-A565-100 | RAB1B Antibody Clinisciences

Western blot analysis of Human HeLa and 293Trap cell lysates showing detection of 22.1 kDa RAB1B protein using Rabbit Anti-RAB1B Polyclonal Antibody (ASM10544). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human HeLa and 293Trap cell lysates. Load: 15 µg . Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-RAB1B Polyclonal Antibody (ASM10544) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 22.1 kDa.

Product Information
Application Applications Legend: WB=Western Blot IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin-embedded Sections) IHC-F=Immunohistochemistry (Frozen Sections) IF=Immunofluorescence FC=Flow Cytopmetry IC=Immunochemistry ICC=Immunocytochemistry E=ELISA IP=Immunoprecipitation DB=Dot Blot CHIP=Chromatin Immunoprecipitation FA=Fluorescence Assay IEM=Immuno electron microscopy EIA=Enzyme Immunoassay	WB, ICC
Primary Accession	Q9H0U4
Other Accession	NP_112243.1
Host	Rabbit
Reactivity	Human, Rat
Clonality	Polyclonal
Description	Rabbit Anti-Human RAB1B Polyclonal
Target/Specificity	Detects ~48 kDa.
Other Names	Ras-related protein Rab-1B Antibody, RAB1B Antibody
Immunogen	Synthetic peptide from the C-terminal of Human RAB1B (aa. 179-189)
Purification	Peptide Affinity Purified
Storage	-20ºC
Storage Buffer	PBS, 50% glycerol, 0.09% sodium azide
Shipping Temperature	Blue Ice or 4ºC
Certificate of Analysis	A 1:1000 dilution of SPC-684 was sufficient for detection of RAB1B in 15 µg of Human HeLa Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization	Cytoplasm \| Membrane \| Lipid-Anchor \| Cytoplasmic Side \| Preautophagosomal Structure Membrane \| Lipid-Anchor \| Cytoplasmic Side

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