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- Specifications
Product Description
Rabbit Fab polyclonal antibody raised against human IgG (H&L).
Immunogen
Human IgG whole molecule.
Host
Rabbit
Reactivity
Human
Specificity
IgG (H&L). Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum. No reaction was observed against anti-papain or anti-rabbit IgG F(c).
Form
Liquid
Conjugation
Unconjugated
Purification
This antibody was prepared from monospecific antiserum by immunoaffinity chromatography using human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation.
Isotype
IgG Fab
Recommend Usage
ELISA (1:30000)
Immunohistochemistry (1:1000-1:5000)
Western Blot (1:2000-1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2 (0.01% (w/v) sodium azide).
Storage Instruction
Store vial at 4°C prior to opening. This product is stable at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Applications
Western Blot
Immunohistochemistry
Enzyme-linked Immunoabsorbent Assay