• Specifications

    Product Description

    Rabbit Fab polyclonal antibody raised against human IgG (H&L).

    Immunogen

    Human IgG whole molecule.

    Host

    Rabbit

    Reactivity

    Human

    Specificity

    IgG (H&L). Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum. No reaction was observed against anti-papain or anti-rabbit IgG F(c).

    Form

    Liquid

    Conjugation

    Unconjugated

    Purification

    This antibody was prepared from monospecific antiserum by immunoaffinity chromatography using human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation.

    Isotype

    IgG Fab

    Recommend Usage

    ELISA (1:30000)
    Immunohistochemistry (1:1000-1:5000)
    Western Blot (1:2000-1:10000)
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2 (0.01% (w/v) sodium azide).

    Storage Instruction

    Store vial at 4°C prior to opening. This product is stable at 4°C as an undiluted liquid. Dilute only prior to immediate use.

    Note

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

  • Applications

    Western Blot

    Immunohistochemistry

    Enzyme-linked Immunoabsorbent Assay