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> Rat IgG (H&L) antibody

Rat IgG (H&L) antibody

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Referência orb347799-2mg

Tamanho : 2mg

Marca : Biorbyt

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    Rat IgG (H&L) antibody

    Catalog Number: orb347799

    Catalog Numberorb347799
    CategoryAntibodies
    DescriptionRat IgG (H&L) antibody
    Species/HostGoat
    ClonalityPolyclonal
    Tested applicationsELISA, IHC, WB
    ReactivityRat
    IsotypeIgG
    ImmunogenRat IgG whole molecule
    Concentration2.0 mg/mL
    Dilution rangeELISA: 1:20,000-1:100,000, IHC: 1:1,000-1:5,000, WB: 1:2,000-1:10,000
    Form/AppearanceLiquid (sterile filtered)
    PurityAnti-RAT IgG (H&L) (GOAT) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgG and Rat Serum.
    ConjugationUnconjugated
    StorageStore vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
    Buffer/Preservatives0.01% (w/v) Sodium Azide
    Alternative namesGoat anti-rat IgG, Rat antibody, rat antibodies, i
    NoteFor research use only
    Application notesAnti-RAT IgG (H&L) (GOAT) Antibody has been tested by ELISA and western blot and is suitable for Immunohistochemistry applications. Specific conditions for reactivity should be optimized by the end user.
    Expiration Date12 months from date of receipt.
    Rat IgG (H&L) antibody

    Western Blot of Anti-Rat IgG (H&L) (GOAT) Antibody (p/n orb347799). Lane M: 3 μl Molecular Ladder. Lane 1: Rat IgG whole molecule. Lane 2: Rat IgG F(c) Fragment (p/n orb346338). Lane 3: Rat IgG Fab Fragment (p/n orb346340). Lane 4: Rat IgM Whole Molecule (p/n orb346341). Lane 5: Rat Serum. All samples were reduced. Load: 50 ng per lane. Block: orb348637 for 30 min at RT. Primary Antibody: Anti-Rat IgG (H&L) (GOAT) Antibody (p/n orb347799) 1:1000 for 60 min at RT. Secondary Antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40000 in orb348637 for 30 min at RT. Predicted/Observed Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.