PH1010-.1 | Recombinant Human TNF-alpha/TNFSF2 Clinisciences

Background

Tumor necrosis factor alpha (TNF-α), also called cachectin, is the best-know member of the TNF-family, which can cause cell death. This protein is produced by neutrophils, activated lymphocytes, macrophages, NK cells, LAK cells, astrocytes endothelial cells, smooth muscle cells and some transformed cells. TNF-α occurs as a secreted, soluble form and as a membrane-anchored form, both of which are biologically active. The naturally-occurring form of TNF-α is glycosylated, but non-glycosylated recombinant TNF-α has comparable biological activity. The biologically active native form of TNF-α is reportedly a trimer. Human and murine TNF-α show approximately 79 % homology at the amino acid level and cross-reactivity between the two species. Two types of receptors for TNF-α have been described and virtually all cell types studied show the presence of one or both of these receptor types.

Description

Alternate Names	Tumor Necrosis Factor-alpha, Human TNF-α, rHuTNF-α/TNFSF2, Cachectin, Differentiation-inducing factor, DIF
Gene ID	7124
Accession #	P01375
AA Sequence	MVRSSSRTPS DKPVAHVVAN PQAEGQLQWL NRRANALLAN GVELRDNQLV VPSEGLYLIY SQVLFKGQGC PSTHVLLTHT ISRIAVSYQT KVNLLSAIKS PCQRETPEGA EAKPWYEPIY LGGVFQLEKG DRLSAEINRP DYLDFAESGQ VYFGIIAL
Molecular Weight	Approximately 17.5 kDa, a single non-glycosylated polypeptide chain containing 158 amino acids.
Source	Escherichia coli
Biological Activity	Fully biologically active when compared to standard. The ED₅₀ as determined by a cytotoxicity assay using murine L929 cells is less than 0.05 ng/ml, corresponding to a specific activity of > 2.0 × 10⁷ IU/mg in the presence of actinomycin D.

Quality Control

Protocol

Components and Storage

Components	10 μg	50 μg	100 μg	1 mg
Recombinant Human TNF-alpha/TNFSF2 (1 mg/mL)	10 μL	50 μL	100 μL	1 mL
Store the components at -20 °C.

Recombinant Human TNF-alpha/TNFSF2

Referência PH1010-.1

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Background

Description

Quality Control

Components and Storage