Recombinant Mouse GM-CSF Protein（E. coli）

Biological Activity

Granulocyte macrophage colony stimulating factor (GM-CSF) was originally thought to be a growth factor capable of supporting granulocyte - macrophage progenitor cell colony formation in vitro. Granulocyte macrophage colony stimulating factor (GM-CSF) is produced by many different cell types, including activated T cells, B cells, macrophages, mast cells, endothelial cells, and fibroblasts, in response to immune and inflammatory cytokines. In addition, granulocyte macrophage colony stimulating factor (GM-CSF) stimulates the proliferation of a variety of tumor cell lines, including osteosarcoma, carcinoma, and adenocarcinoma cell lines.

Apparent molecular weight: ~14.3 kDa, reduced by SDS-PAGE

Chemical Information

Solubility (25°C)	Reconstitute the lyophilized powder in ddH2O or PBS up to 100 μg/ml.
Storage	Stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days and at -20°C for 3 months.

References

[1] Elektra Kantzari Robinson, et al. PLoS One. Generation and utilization of a HEK-293T murine GM-CSF expressing cell line

[2] Michael Dougan, et al. Immunity. GM-CSF, IL-3, and IL-5 Family of Cytokines: Regulators of Inflammation

[3] Marco Grasse, et al. Vaccine. GM-CSF improves the immune response to the diphtheria-component in a multivalent vaccine

[4] Qingjie Xue, et al. Hum Vaccin Immunother. Efficacy of recombinant adenovirus expressing a fusion gene from GM-CSF and Epstein-Barr virus LMP2A in a mouse tumor model

[5] B L Liu, et al. Gene Ther. ICP34.5 deleted herpes simplex virus with enhanced oncolytic, immune stimulating, and anti-tumour properties