Recombinant Sortase A Protease

Recombinant Sortase A Protease
Catalog number: B2011493
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 10 µg
Molecular Weight or Concentration: 50 µg/mL
Supplied as: Solution
Applications: molecular tool for various biochemical applications
Storage: -80 °C
Keywords: Sortase A protease, recombinant
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

References:
1: Chen I, Dorr BM, Liu DR. A general strategy for the evolution of bond-forming enzymes using yeast display Proc Natl Acad Sci U S A. 2011 Jul 12;108(28):11399-404.
2: Stanborough T, Suryadinata R, Fegan N, Powell SM, Tamplin M, Nuttall SD, Chandry PS. Characterisation of the Brochothrix thermosphacta sortase A enzyme FEMS Microbiol Lett. 2018 Sep 1;365(17).
3: Sarpong K, Bose R. Efficient sortase-mediated N-terminal labeling of TEV protease cleaved recombinant proteins Anal Biochem. 2017 Mar 15;521:55-58.
4: Westerlund K, Myrhammar A, Tano H, Gestin M, Karlström AE. Stability Enhancement of a Dimeric HER2-Specific Affibody Molecule through Sortase A-Catalyzed Head-to-Tail Cyclization Molecules. 2021 May 12;26(10):2874.
5: Bolscher JG, Oudhoff MJ, Nazmi K, Antos JM, Guimaraes CP, Spooner E, Haney EF, Garcia Vallejo JJ, Vogel HJ, van’t Hof W, Ploegh HL, Veerman EC. Sortase A as a tool for high-yield histatin cyclization FASEB J. 2011 Aug;25(8):2650-8.
6: Stanger K, Maurer T, Kaluarachchi H, Coons M, Franke Y, Hannoush RN. Backbone cyclization of a recombinant cystine-knot peptide by engineered Sortase A FEBS Lett. 2014 Nov 28;588(23):4487-96.
7: Wójcik M, Szala K, van Merkerk R, Quax WJ, Boersma YL. Engineering the specificity of Streptococcus pyogenes sortase A by loop grafting Proteins. 2020 Nov;88(11):1394-1400.
8: Xu Z, Rivera-Hernandez T, Chatterjee O, Walker MJ, Moyle PM. Semisynthetic, self-adjuvanting vaccine development: Efficient, site-specific sortase A-mediated conjugation of Toll-like receptor 2 ligand FSL-1 to recombinant protein antigens under native conditions and application to a model group A streptococcal vaccine J Control Release. 2020 Jan 10;317:96-108.
9: Jin M, Chen Y, Zhao Y, Che L, Ma Y, Li J, Wang Y, Tao H, Ma J, Pan B, Liu C, Huang P. Sortase A-aided Escherichia coli expression system for functional osteoprotegerin cysteine-rich domain Appl Microbiol Biotechnol. 2017 Jun;101(12):4923-4933.
10: Li H, Chen Y, Zhang B, Niu X, Song M, Luo Z, Lu G, Liu B, Zhao X, Wang J, Deng X. Inhibition of sortase A by chalcone prevents Listeria monocytogenes infection Biochem Pharmacol. 2016 Apr 15;106:19-29.

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