S-Adenosyl methionine ELISA Kit

Referência OKEH02593

Tamanho : 96Wells

Marca : Aviva Systems Biology

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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH02593
Product Info
Predicted Species ReactivityAll Species
ApplicationELISA-Comp
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology S-Adenosyl methionine ELISA Kit (OKEH02593) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-S-Adenosyl methionine antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated S-Adenosyl methionine and incubated. The S-Adenosyl methionine found in the sample or standards competes with the biotinylated S-Adenosyl methionine for limited binding sites on the immobilized anti-S-Adenosyl methionine antibody. Excess unbound biotinylated S-Adenosyl methionine and sample or standard S-Adenosyl methionine is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated S-Adenosyl methionine captured in the well and inversely proportional to the amount of S-Adenosyl methionine which was contained in the sample or standard.
ELISA Kit Range1.56-100nmol/L
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.1% (n = 20)
Mean Inter-assay CV%: < 7.4% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-S-Adenosyl methionine Microplate96 Wells (12 x 8 Well strips)
S-Adenosyl methionine Lyophilized Standard2 x 100 nmol
Sample Diluent1 x 20 mL
100X S-Adenosyl methionine-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationTarget Abbr: SAM
Target Name: S-Adenosyl methionine
::Pubchem: 34756
::Chemical formula: C15H23N6O5S+
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates, cell culture supernatants and other biological fluids
Sensitivity0.38nmol/L
SpecificityNatural and recombinant General S-Adenosyl methionine
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Alias SymbolsSAM, S-Adenosyl methionine, SAM
Protein NameS-Adenosyl methionine
Description of TargetS-Adenosyl methionine (ademetionine, AdoMet, SAM, SAMe, SAM-e) is a common cosubstrate involved in methyl group transfers. SAM was first discovered in Italy by G. L. Cantoni in 1952. It is made from adenosine triphosphate (ATP) and methionine by methionine adenosyltransferase (EC 2.5.1.6). Transmethylation, transsulfuration, and aminopropylation are the metabolic pathways that use SAM. Although these anabolic reactions occur throughout the body, most SAM is produced and consumed in the liver. The methyl group (CH3) attached to the methionine sulfur atom in SAM is chemically reactive. This allows donation of this group to an acceptor substrate in transmethylation reactions. More than 40 metabolic reactions involve the transfer of a methyl group from SAM to various substrates, such as nucleic acids, proteins, lipids and secondary metabolites. In bacteria, SAM is bound by the SAM riboswitch, which regulates genes involved in methionine or cysteine biosynthesis.

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MET-5152
 96assays