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> SARS-CoV-2 (COVID-19) Spike RBD Protein, P.1 / Gamma variant, His tag (active)

SARS-CoV-2 (COVID-19) Spike RBD Protein, P.1 / Gamma variant, His tag (active)

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Referência GTX136043-pro

Tamanho : 100ug

Marca : GeneTex

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SARS-CoV-2 (COVID-19) Spike RBD Protein, P.1 / Gamma variant, His tag (active). GTX136043-pro
SARS-CoV-2 (COVID-19) Spike RBD Protein, P.1 / Gamma variant, His tag (active). GTX136043-pro
SARS-CoV-2 (COVID-19) Spike RBD Protein, P.1 / Gamma variant, His tag (active). GTX136043-pro
SARS-CoV-2 (COVID-19) Spike RBD Protein, P.1 / Gamma variant, His tag (active). GTX136043-pro
SARS-CoV-2 (COVID-19) Spike RBD Protein, P.1 / Gamma variant, His tag (active). GTX136043-pro

Application

 Binding Assay, WB, ELISA

Species

 SARS Coronavirus 2
Package
100 μg
Datasheet File
View product citations for antibody GTX136043-pro on CiteAb

APPLICATION

Application Note

Capture : GTX635807, Detection : GTX635791-01

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with distilled water to 0.8 mg/ml. Lyophilized from PBS, 2% Trehalose, 5% Mannitol, 0.01% Tween-80.

Preservative

No Preservative

Storage

Store at 4ºC or below. After reconstitution, keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. For long-term storage after reconstitution, aliquot and store at -70ºC or below. Do not vortex.

Region/Sequence

SARS-CoV-2 Spike RBD of QHD43416.1 (319-541 a.a) with K417T, E484K, and N501Y mutations and His tag at the C-terminus

Expression System

HEK293 cells

Purity

>95%

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Gamma variant Spike , P.1 Spike , SARS-CoV-2 Gamma variant Spike , COVID-19 Gamma variant Spike

DATA IMAGES

SARS-CoV-2 (COVID-19) Spike RBD Protein, P.1 / Gamma variant, His tag (active). GTX136043-pro

GTX136043-pro ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike RBD Protein, P.1 / Gamma variant, His tag (active) (GTX136043-pro) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] (HRP) (GTX635791-01) (1 μg/mL)

SARS-CoV-2 (COVID-19) Spike RBD Protein, P.1 / Gamma variant, His tag (active). GTX136043-pro

GTX136043-pro Binding Assay Image

Functional ELISA analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike RBD (N501Y Mutant) protein, His tag (active) (GTX136014-pro), SARS-CoV-2 (COVID-19) Spike RBD (K417N, E484K, N501Y Mutant) protein, His tag (active) (GTX136022-pro), SARS-CoV-2 (COVID-19) Spike RBD (K417T, E484K, N501Y Mutant) protein, His tag (active) (GTX136043-pro), SARS-CoV-2 (COVID-19) Spike RBD (E484K, N501Y Mutant) protein, His tag (active) (GTX136058-pro) and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (13-10000 ng/mL). Bound protein was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

SARS-CoV-2 (COVID-19) Spike RBD Protein, P.1 / Gamma variant, His tag (active). GTX136043-pro

GTX136043-pro Binding Assay Image

Inhibition analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike RBD (N501Y Mutant) protein, His tag (active) (UK variant) (GTX136014-pro), SARS-CoV-2 (COVID-19) Spike RBD (K417N, E484K, N501Y Mutant) protein, His tag (active) (South Africa variant) (GTX136022-pro), SARS-CoV-2 (COVID-19) Spike RBD (K417T, E484K, N501Y Mutant) protein, His tag (active) (Brazil variant) (GTX136043-pro), SARS-CoV-2 (COVID-19) Spike RBD (E484K, N501Y Mutant) protein, His tag (active) (UK variant) (GTX136058-pro) and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX136090-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (1000 ng/mL). ACE2 binding was inhibited by increasing concentrations of SARS-CoV-2 (COVID-19) Spike RBD antibody (GTX135709) (13.72-10000 ng/mL). Bound ACE2 was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

SARS-CoV-2 (COVID-19) Spike RBD Protein, P.1 / Gamma variant, His tag (active). GTX136043-pro

GTX136043-pro Image

5 μg of GTX136043-pro SARS-CoV-2 (COVID-19) Spike RBD (K417T, E484K, N501Y), His tag protein was analyzed using SDS-PAGE and stained with coomassie blue and captured by monochrome camera.

SARS-CoV-2 (COVID-19) Spike RBD Protein, P.1 / Gamma variant, His tag (active). GTX136043-pro

GTX136043-pro WB Image

SARS-CoV-2 (COVID-19) Spike RBD (K417T, E484K, N501Y), His tag protein (GTX136043-pro, 0.2 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with the SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] (GTX635692) diluted at 1:5000.

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REVIEW

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