SARS-CoV-2 Nucleocapsid Protein

Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2/COVID-19) Nucleocapsid Protein is a recombinant protein expressed in E. coli.

The SARS-CoV-2 Nucleoprotein (also known as Nucleocapsid Protein or N Protein) is a protein that binds to the RNA in the viral particle. Changes to two amino acids in the nucleoprotein are thought to contribute to the virus’ ability to infect humans; this mutation allows the virus to reduce the binding capability of an HLA-C allele found in many Europeans. The nucleoprotein is the second most-common protein in SARS-CoV-2 targeted by the immune system, after the spike protein.

Target	Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Nucleocapsid Protein
Origin	Virus
Expression	Recombinant
Tested Applications	ELISA, WB, SDS-PAGE
Host	E. coli
Recommended dilutions	Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Form	Liquid
Purity	> 95% (SDS-PAGE)
Purification	0.2 µm filtered.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
NCBI Accession	QHD43423.2
Sequence Fragment	Met1-Ala419
Sequence	MSDNGPQNQR NAPRITFGGP SDSTGSNQNG ERSGARSKQR RPQGLPNNTA SWFTALTQHG KEDLKFPRGQ GVPINTNSSP DDQIGYYRRA TRRIRGGDGK MKDLSPRWYF YYLGTGPEAG LPYGANKDGI IWVATEGALN TPKDHIGTRN PANNAAIVLQ LPQGTTLPKG FYAEGSRGGS QASSRSSSRS RNSSRNSTPG SSRGTSPARM AGNGGDAALA LLLLDRLNQL ESKMSGKGQQ QQGQTVTKKS AAEASKKPRQ KRTATKAYNV TQAFGRRGPE QTQGNFGDQE LIRQGTDYKH WPQIAQFAPS ASAFFGMSRI GMEVTPSGTW LTYTGAIKLD DKDPNFKDQV ILLNKHIDAY KTFPPTEPKK DKKKKADETQ ALPQRQKKQQ TVTLLPAADL DDFSKQLQQS MSSADSTQA
Tag	N-terminal 6 His tag
Buffer	20 mM Tris-HCl, 300 mM NaCl, 10% glycerol, 1 mM EDTA, pH 8.0.
Endotoxin Level	< 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
Availability	Shipped within 7-12 working days.
Note	This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use.

Research Articles on Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Nucleocapsid Protein