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> SARS-CoV-2 Spike Protein S1 RBD ELISA Kit

SARS-CoV-2 Spike Protein S1 RBD ELISA Kit

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Referência E-EL-E605-96T

Tamanho : 96T

Marca : Elabscience

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Product Summary
Sensitivity 0.09 ng/mL
Detection Range 0.39-25 ng/mL
Sample Volume 100 μL
Total Assay Time 3 h 30 min
Reacitivity Universal
Specificity This kit recognizes Human S1 RBD in samples.No significant cross-reactivity or interference between Human S1 RBD and analogues was observed
Recovery 80%-120%
Sample Type serum, plasma and other biological fluids
Detection Method Colorimetric method, ELISA, Sandwich
Assay Type Sandwich-ELISA
Size 96T / 48T / 24T / 96T*5 / 96T*10
Storage 2-8℃/-20℃
Expiration Date 6 months
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to SARS-CoV-2 S1RBD. Samples (or Standards) and biotinylated detection antibody specific for SARS-CoV-2 S1RBD are added to the micro ELISA plate wells. SARS-CoV-2 S1RBD would combine with the specific antibody. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain SARS-CoV-2 S1RBD, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of SARS-CoV-2 S1RBD. You can calculate the concentration of SARS-CoV-2 S1RBD in the samples by comparing the OD of the samples to the standard curve.
Uniport ID P0DTC2
Research Area SARS-CoV-2 , COVID 19
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