SCLC-24H
Marca : CLS Cell Lines Service
SCLC-24H Cells
General information
Organism | Human |
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Tissue | Lung |
Disease | Small cell carcinoma |
Metastatic site | Pleural effusion |
Synonyms | MAR-24H, MAR 24H, MAR24H, 24H |
Characteristics
Age | 46 years |
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Gender | Male |
Ethnicity | Caucasian |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | SCLC-24H (Cytion catalog number 300177) |
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Biosafety level | 1 |
Expression / Mutation
Handling
Culture Medium | DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile | Amelogenin: x,x |
HLA alleles | A*: 01:01:01, 32:01:01 B*: 27:05:02, 51:01:01 C*: 02:02:02 DRB1*: 04:01:01, 09:01:02G DQA1*: 03:01:01, 03:02:01 DQB1*: 03:02:01, 03:03:02 DPB1*: 02:01:02, 04:01:01 E: 01:01:01 |