SiO2 with carboxyl (COOH) surface groups for the covalent attachment of biomolecules. Carboxyl silica microspheres are sold in 1.0g amounts. Supplied dry, they are available in four nominal mean diameters: ~0.5µm, ~1.0µm, ~2.0µm, and ~5.0µm.
TechNote 104, Silica Microspheres
PDS 702, Silica Microspheres
SDS SG141 (Functionalized Silica)
| Catalog Number | Nominal Diameter | Specification Range |
|---|---|---|
| SC03000 | 0.5µm | 0.46 - 0.54µm |
| SC04000 | 1.0µm | 0.95 - 1.05µm |
| SC05000 | 2.0µm | 2.80 - 2.20µm |
| SC05001 | 5.0µm | 4.80 - 5.20µm |
References
Wang L, Yang C, Tan W. (2004) Dual-luminophore-doped silica nanoparticles for multiplexed signaling. Nano Lett; 5(1):37-43.
Lauer, SA, Nolan, JP (2002) Development and characterization of Ni-NTA-bearing microspheres. Cytometry, 48: 136–145.
Yoo CE, Moon HS, Kim YJ, Park JM, Park D, Han KY,…Park WY (2015) Highly dense, optically inactive silica microbeads for the isolation and identification of circulating tumor cells. Biomaterials Jan;75:271-8. (CTC isolation via anti-EpCAM coated silica microspheres)
Litvinov, J, Moen, S. T, Koh, CY, & Singh, AK (2016). Centrifugal sedimentation immunoassays for multiplexed detection of enteric bacteria in ground water. Biomicrofluidics, 10(1), 014103.(5µm carboxyl silica microspheres)
Litvinov J, Moen ST, Berry GJ, Loeffelholz M, Singh AK, & Koh CY. (2017). Centrifugal Microfluidic Platform for Rapid, Multiplexed Detection of TB and HIV Biomarkers in Whole Blood Samples. Journal of Bioengineering & Biomedical Science, 6(SAND-2015-11159J).(SC05001, 5µm Silica-COOH microspheres)
Iswardy E, Tsai TC, Cheng IF, Ho TC, Perng GC, & Chang HC. (2017). A bead-based immunofluorescence-assay on a microfluidic dielectrophoresis platform for rapid dengue virus detection. Biosensors and Bioelectronics, 95, 174-180.(Anti-flavivirus antibody coupled to 1µm Silica COOH [Cat. SC04000] via PolyLink Coupling Kit [Cat. PL01N])