SK-Mel-1
Referência C0020005
Tamanho : OneFrozenvial
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Disease:
Malignant melanoma
Origin:
This cell line was isolated using cells obtained from the thoracic duct of a patient with widespread and rapidly progressing malignant melanoma.
Species:
Homo sapiens, human Malignant melanoma; skin; derived from metastatic site: lymphatic system Melanoma Spherical, clusters in suspension, dark color pellet after centrifugation. Male, Caucasian, 29 years of age. AddexBio-formulated EMEM (C0005-01) + 10% FBS Cultures can be maintained by addition or replacement of fresh medium. Establish new cultures at 1 x 10^5 cells/mL and maintain at 2 x 10^5 cells/mL. Allow the flask to sit at room temperature (or at 37°C). Culture at 5% CO2, 37°C. Renew medium once every 2 to 3 days depending on cell density. Complete growth medium supplemented with 5% (v/v) DMSO I Bacteria: Negative Yeast: Negative Mycoplasma: Negative HIV: Negative Hepatitis B: Negative Amelogenin: X,Y CSF1PO: 12,13 D13S317: 11 D16S539: 11,12 D5S818:12,13 D7S820: 12 THO1:6 TPOX: 11 vWA: 16,17 If use of this culture results in a scientific publication, it should be cited in the publication as: SK-MEL-1 (AddexBio C0020005) Tissue:
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Citation Format:
References for SK-MEL-1 cells:
1. Oettgen HF, Aoki T, Old LJ, Boyse EA, de Harven E, Mills GM. Suspension culture of a pigment-producing cell line derived from a human malignant melanoma. J Natl Cancer Inst. 1968;41(4):827-843. PMID: 4879578