SK-MEL-28 growing culture
Referência 330337
Tamanho : 1cellcultureflask
Marca : CLS Cell Lines Service
SK-MEL-28 Cells
Key points about the SK-MEL-28 cell line
Description | This cell line was established from an axillary lymph node of a 51-year-old male of unknown ethnicity by T.Takahashi and associates who have isolated this cell line in a series of melanoma lines. |
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Organism | Human |
Tissue | Skin |
Disease | Cutaneous melanoma |
Synonyms | SK-Mel-28, SK.MEL.28, SK-MEL 28, SK MEL-28, SK MEL 28, SK Mel 28, SKMel-28, SKMEL-28, SK-MEL28, SK-Mel28, SK Mel28, SKMEL28, SKMel28, SKmel28, SKML-28, SK28, AU-Mel, P-36, P36 |
Details
Age | 51 years |
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Gender | Male |
Morphology | Polygonal |
Growth properties | Adherent |
Specifications
Citation | SK-MEL-28 (Cytion catalog number 300337) |
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Biosafety level | 1 |
Genetic profile of the human melanoma cell line SK-MEL-28
Protein expression | p53 positive |
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Isoenzymes | PGM3, 1, PGM1, 1, ES-D, 1, AK-1, 1-2, GLO-1, 2, G6PD, B |
Tumorigenic | Yes, in nude mice. Forms malignant melanoma (large round cell type) |
Products | Melanin |
Mutational profile | BRAF V600E mut: V600E type BRAF Mutation was determined by DNA based methods (sequencing, RT-PCR) and protein based methods (Western Blot), N-Ras wt |
Maintenance techniques
Culture Medium | DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:3 is recommended |
Fluid renewal | 2 to 3 times per week |
Freezing recovery | Leave at least 48 hours post to thawing until removal of medium or subculture |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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SKMEL28 cell purity and identity checks
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile | Amelogenin: x,y CSF1PO: 10,12 D13S317: 11,12 D16S539: 9,12 D5S818: 13 D7S820: 10 TH01: 7 TPOX: 8,12 vWA: 16,19 D3S1358: 16,18 D21S11: 28,29 D18S51: 12,16 Penta E: 8,12 Penta D: 9,1 D8S1179: 13 FGA: 19 |
HLA alleles | A*: 11:01:01 B*: 40:01:02 C*: 03:04:01 DRB1*: 04:04:01 DQA1*: 03:01:01 DQB1*: 03:02:01 DPB1*: 03:01:01 E: 01:03:02 |