Disease:

Squamous cell carcinoma

Origin:

Derived from Metastatic Site: pleural effusion.

Species:

Homo sapiens, human

Tissue:

Lung

Morphology:

Epithelial

Properties:

Adherent

Cytogenic data:

The stemline chromosome number is hypotriploid, with the 2S component occurring at 3.2%. Seventeen to 20 marker chromosomes were common to most S metaphases. Normal X, 13, and 19 chromosomes were absent, and chromosomes 2, 3, 14, 17 and 20 were generally monosomic. The Y chromosome was not detected using QM staining.

Patient:

Caucasian, male, 65 years of age.

Medium:

AddexBio-formulated EMEM (C0005-01) + 10% FBS

Subculture:

Subculture at a ratio of 1:3 to 1:6 using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37°C.

Freezing Medium:

Complete growth culture medium supplemented with 5% (v/v) DMSO