Syrian Hamster monoclonal antibody PE/Cy5 labled to CD28
Referência CFG8787-50
Tamanho : 50Assays
Marca : Cohesion Biosciences
Product Name | Anti-CD28 Antibody-PE/Cy5 labled |
Cat No: | CFG8787 |
Source: | Syrian Hamster |
Reactivity: | M |
Applications: | IF, FC |
*Application Key: | |
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference | |
*Species Reactivity Key: | |
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish |
Description: Syrian Hamster monoclonal antibody PE/Cy5 labled to CD28 Immunogen: C57BL/6 mouse T-cell lymphoma EL-4 Purification: The antibody was purified by affinity chromatography. Clonality: Monoclonal (clone: 37.51) Conjugation: PE/Cy5 Form: Syrian Hamster IgG. Liquid in PBS, pH 7.3, 0.2% BSA, and 0.02% sodium azide. Dilution: 10 μl / assay Gene Symbol: CD28 Alternative Names: T-cell-specific surface glycoprotein CD28; TP44; CD28Entrez Gene (Mouse) : SwissProt (Mouse) : Directions for Use : 1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add 10 μl labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 ml RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).Storage/Stability : Shipped and store at 4°C for one year. Do not freeze.