Tissue, Universal RNA, Rat Adult Normal

Description:|Rat Universal RNA is prepared from several male and female Sprague Dawley or Wistar rat|whole bodies without fur. This total RNA serves as a standard for comparison from|microarray studies.||Quality Control:|1. The integrity of the RNA is examined by visual inspection for the presence of intact bands of 18s and 28s ribosomal RNA when electrophoreses on a denaturing agarose gel. The quality and purity of total RNA were tested by spectrophotometer. A260/280 is between 1.8 and 2.0 (detected in 10mM Tris-HCl, pH 7.5).|2. The Universal RNA is analyzed by capillary electrophoresis with Agilent 2100 bioanalyzer. RNA integrity is determined with RIN (RNA Integrity Number)≥7, and the area of 28s over 18s ratio ≥ 1.|3. The RNA is treated by DNase I, and is tested as DNA free RNA by PCR.|4. cDNA synthesis is successfully performed by using this RNA as template.|5. Universal RNA is labeled and hybridized to a cDNA microarray, expected result of gene expression is consistent with each lot.||Storage and Stability:|Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.