TMEM106B (NM_018374) Human Tagged ORF Clone
Referência RC206257
Tamanho : 10ug
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TMEM106B (NM_018374) Human Tagged ORF Clone
| Product Data | |
| Target Symbol | TMEM106B |
|---|---|
| Synonyms | HLD16 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | ORF Nucleotide Sequence >RC206257 ORF sequence Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAAAGTCTCTTTCTCATTTGCCTTTGCATTCAAGCAAAGAAGATGCTTATGATGGAGTCACATCTG AAAACATGAGGAATGGACTGGTTAATAGTGAAGTCCATAATGAAGATGGAAGAAATGGAGATGTCTCTCA GTTTCCATATGTGGAATTTACAGGAAGAGATAGTGTCACCTGCCCTACTTGTCAGGGAACAGGAAGAATT CCTAGGGGGCAAGAAAACCAACTGGTGGCATTGATTCCATATAGTGATCAGAGATTAAGGCCAAGAAGAA CAAAGCTGTATGTGATGGCTTCTGTGTTTGTCTGTCTACTCCTTTCTGGATTGGCTGTGTTTTTCCTTTT CCCTCGCTCTATCGACGTGAAATACATTGGTGTAAAATCAGCCTATGTCAGTTATGATGTTCAGAAGCGT ACAATTTATTTAAATATCACAAACACACTAAATATAACAAACAATAACTATTACTCTGTCGAAGTTGAAA ACATCACTGCCCAAGTTCAATTTTCAAAAACAGTTATTGGAAAGGCACGCTTAAACAACATAACCATTAT TGGTCCACTTGATATGAAACAAATTGATTACACAGTACCTACCGTTATAGCAGAGGAAATGAGTTATATG TATGATTTCTGTACTCTGATATCCATCAAAGTGCATAACATAGTACTCATGATGCAAGTTACTGTGACAA CAACATACTTTGGCCACTCTGAACAGATATCCCAGGAGAGGTATCAGTATGTCGACTGTGGAAGAAACAC AACTTATCAGTTGGGGCAGTCTGAATATTTAAATGTACTTCAGCCACAACAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence >RC206257 protein sequence Red=Cloning site Green=Tags(s) MGKSLSHLPLHSSKEDAYDGVTSENMRNGLVNSEVHNEDGRNGDVSQFPYVEFTGRDSVTCPTCQGTGRI PRGQENQLVALIPYSDQRLRPRRTKLYVMASVFVCLLLSGLAVFFLFPRSIDVKYIGVKSAYVSYDVQKR TIYLNITNTLNITNNNYYSVEVENITAQVQFSKTVIGKARLNNITIIGPLDMKQIDYTVPTVIAEEMSYM YDFCTLISIKVHNIVLMMQVTVTTTYFGHSEQISQERYQYVDCGRNTTYQLGQSEYLNVLQPQQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms | Chromatograms Sequencher program is needed, download here |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
| ACCN | NM_018374 |
| ORF Size | 822 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_018374.4 |
| RefSeq Size | 6514 bp |
| RefSeq ORF | 825 bp |
| Locus ID | 54664 |
| UniProt ID | Q9NUM4 |
| Cytogenetics | 7p21.3 |
| Protein Families | Transmembrane |
| MW | 31.1 kDa |
| Summary | Involved in dendrite morphogenesis and maintenance by regulating lysosomal trafficking via its interaction with MAP6. May act by inhibiting retrograde transport of lysosomes along dendrites. Required for dendrite branching.[UniProtKB/Swiss-Prot Function] |
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