Reagentes e instrumentos para imunologia, biologia celular e biologia molecular.

Total Bile Acid (TBA) Colorimetric Assay Kit

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Referência E-BC-K181-M-96T

Tamanho : 96T

Contactar o distribuidor local :

Telefone : +1 850 650 7790

Marca : Elabscience

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Contactar o distribuidor local :

Telefone : +1 850 650 7790

Detection Principle	With S-NAD⁺ as hydrogen receptor, 3α-hydroxy steroid dehydrogenase catalyzed the dehydrogenation of bile acids to produce 3-ketone steroids, transforming S-NAD⁺ into S-NADH. Meanwhile, NADH was used as hydrogen donor. 3α-hydroxy steroid dehydrogenase catalyzed the production of bile acids from 3-ketone steroids. Through the enzyme cycle reaction, S-NADH is continuously generated, which has the maximum absorption peak at 405 nm. Measure the OD value at 405 nm and the changes of absorbance is proportional to the concentration of bile acid.
Synonyms	TBA
Sample Type	Serum,Animal tissue
Detection Method	Colorimetric method
Detection Instrument	Microplate reader (400-410 nm,optimum wavelength: 405 nm)
Research Area	Liver And Renal Function
Other Reagents Required	Normal saline (0.9% NaCl)
Storage	This product can be stored at 2-8°C for 12 months with shading light.
Valid Period	12 months
Sensitivity	1.36 μmol/L
Detection Range	1.77-40 μmol/L
Precision	inter-assay CV: 3.7% \| intra-assay CV: 3.4%
Assay Time	25 min

Cat.No.	Product Name	Sizes
E-BC-K235-M	Alanine Aminotransferase (ALT/GPT) Activity Assay Kit	96T , 48T
E-BC-K236-M	Aspartate Aminotransferase (AST/GOT) Activity Assay Kit	96T , 48T
E-BC-K760-M	Total Bilirubin (TBIL) Colorimetric Assay Kit	96T , 48T
E-BC-K761-M	Direct Bilirubin (DBIL) Colorimetric Assay Kit	96T , 48T

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type	Dilution Factor
Human serum	1
Dog serum	1
Mouse serum	1
Rat serum	1
Bovine serum	1
10% mouse liver	1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.