TREM2 antibody [HL1738]
Referência GTX637386-25ul
Tamanho : 25ul
Marca : GeneTex
Host | Rabbit |
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Clonality | Monoclonal |
Clone Name | HL1738 |
Isotype | IgG |
Application | WB, ICC/IF, IHC-P, ELISA, Sandwich ELISA |
Reactivity | Human |
Package | 100 μl, 25 μl |
APPLICATION
Application Note
Application | Recommended Dilution |
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WB | 1:500-1:3000 |
ICC/IF | Assay dependent |
IHC-P | Assay dependent |
ELISA | Assay dependent |
Sandwich ELISA | Assay dependent |
Note : Sandwich ELISACapture : GTX637387, Detection : GTX637386 or Capture : GTX637386, Detection : GTX637387 The detection antibody requires HRP conjugation. Please contact us for the conjugation service. |
Calculated MW
PROPERTIES
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Antigen Species
Immunogen
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Note
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
Background
Database
DATA IMAGES
GTX637386 ICC/IF ImageTREM2 antibody [HL1738] detects TREM2 protein at cell membrane and cytoplasm by immunofluorescent analysis.Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: TREM2 stained by TREM2 antibody [HL1738] (GTX637386) diluted at 1:250.Blue: Fluoroshield with DAPI (GTX30920). |
GTX637386 ELISA ImageIndirect ELISA analysis was performed by coating the plate with recombinant E.coli expressed, full-length human TREM2 protein (1215.95-19.07 pM). Coated protein was probed with TREM2 antibody [HL1738] (GTX637386) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody. |
GTX637386 ELISA ImageSandwich ELISA detection of recombinant E.coli expressed, full-length human TREM2 protein using antibodies as below. Capture: TREM2 antibody [HL1739] (GTX637387) (5 μg/mL) Detection: HRP-conjugated TREM2 antibody [HL1738] (GTX637386) (1 μg/mL)Please notice that GTX637386 needs to be conjugated to HRP to function as the detection antibody when paired with GTX637387. Please contact us for custom HRP-conjugated antibody. |
GTX637386 ELISA ImageSandwich ELISA detection of recombinant E.coli expressed, full-length human TREM2 protein using antibodies as below. Capture: TREM2 antibody [HL1738] (GTX637386) (5 μg/mL) Detection: HRP-conjugated TREM2 antibody [HL1739] (GTX637387) (1 μg/mL)Please notice that GTX637387 needs to be conjugated to HRP to function as the detection antibody when paired with GTX637386. Please contact us for custom HRP-conjugated antibody. |
GTX637386 WB ImageVarious whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TREM2 antibody [HL1738] (GTX637386) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program. |
GTX637386 WB ImageNon-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TREM2 antibody [HL1738] (GTX637386) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
GTX637386 IHC-P ImageTREM2 antibody [HL1738] detects TREM2 protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded human lung cancer.TREM2 stained by TREM2 antibody [HL1738] (GTX637386) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min |