Western Blot (Cell lysate)

TYMS monoclonal antibody (M01), clone 3A1 Western Blot analysis of TYMS expression in HeLa ( Cat # L013V1 ).

Western Blot (Transfected lysate)

Western Blot analysis of TYMS expression in transfected 293T cell line by TYMS monoclonal antibody (M01), clone 3A1.

Lane 1: TYMS transfected lysate(35.7 KDa).
Lane 2: Non-transfected lysate.

Western Blot (Recombinant protein)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Immunoperoxidase of monoclonal antibody to TYMS on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml]

Sandwich ELISA (Recombinant protein)

Detection limit for recombinant GST tagged TYMS is approximately 0.03ng/ml as a capture antibody.

ELISA

Immunofluorescence

Immunofluorescence of monoclonal antibody to TYMS on HeLa cell. [antibody concentration 20 ug/ml]

Entrez GeneID

7298

GeneBank Accession#

BC013919

Protein Accession#

AAH13919

Gene Name

TYMS

Gene Alias

HsT422, MGC88736, TMS, TS, TSase

Gene Description

thymidylate synthetase

Omim ID

188350

Gene Ontology

Hyperlink

Gene Summary

Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5,10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of this gene and that of a naturally occuring antisense transcript rTSalpha (GeneID:55556) vary inversely when cell-growth progresses from late-log to plateau phase. [provided by RefSeq

Other Designations

Thymidylate synthase