UGP2 Rabbit Polyclonal Antibody

Referência AP21425AF-N

Tamanho : 10mg

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UGP2 Rabbit Polyclonal Antibody

Specifications
Product Data
Application ELISA, ID, IF, IP, R, WB
Application This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of choice.
Working Dilutions:
Non-precipitating antibody-binding techniques: 1/1,000-1/7,000.
Reactivity Bovine
Antibody Host Rabbit
Clonality Polyclonal
Immunogen Uridine-5’-diphosphoglucose Pyrophosphorylase isolated and purified from Bovine liver.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition.
Cross-reactivities against enzymes of other sources may occur but have not been determined.
Buffer PBS, pH 7.2 without preservatives and foreign proteins
State: Azide Free
State: Lyophilized hyperimmune IgG fraction
Reconstitution Method Restore by adding 1.0 ml of sterile distilled water
Concentration lot specific
Purification Ammonium Sulphate Precipitation and Ion Exchange Chromatography
Conjugation Unconjugated
Storage Store the antibody lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer.
If a slight precipitation occurs upon storage, this should be removed by centrifugation.
Stability Shelf life: one year from despatch.
Database Link
Background UGP2 is an important intermediary in mammalian carbohydrate interconversions. It transfers a glucose moiety from glucose-1-phosphate to MgUTP and forms UDP-glucose and MgPPi. In liver and muscle tissue,UDP-glucose is a direct precursor of glycogen; in lactating mammary gland it is converted to UDP-galactose which is then converted to lactose. The eukaryotic enzyme has no significant sequence similarity to the prokaryotic enzyme.
Synonyms UGP1, UDP-glucose pyrophosphorylase, UGPase, UDPGP
Reference Data
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