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> Vimentin (Mesenchymal Cell Marker) Antibody - With BSA and Azide

Vimentin (Mesenchymal Cell Marker) Antibody - With BSA and Azide

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Referência AH10809-U0100

Tamanho : 100ug

Marca : Abcepta

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  • IHC - Vimentin (Mesenchymal Cell Marker) Antibody - With BSA and Azide AH10809-20
    Formalin-fixed, paraffin-embedded human Melanoma stained with Vimentin Monoclonal Antibody (SPM576).
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, FC
Primary Accession P08670
Other Accession 7431, 455493
Reactivity Human, Pig, Chicken, Goat, Bovine, Dog, Cat
Host Mouse
Clonality Monoclonal
Isotype Mouse / IgG1
Clone Names SPM576
Calculated MW 57-60kDa
Additional Information
Gene ID 7431
Other Names Vimentin, VIM
Format 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
StorageStore at 2 to 8°C.Antibody is stable for 24 months.
Precautions Vimentin (Mesenchymal Cell Marker) Antibody - With BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name VIM (HGNC:12692)
Function Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Plays a role in cell directional movement, orientation, cell sheet organization and Golgi complex polarization at the cell migration front (By similarity). Protects SCRIB from proteasomal degradation and facilitates its localization to intermediate filaments in a cell contact-mediated manner (By similarity).
Cellular Location Cytoplasm. Cytoplasm, cytoskeleton. Nucleus matrix {ECO:0000250|UniProtKB:P31000}. Cell membrane {ECO:0000250|UniProtKB:P20152}
Tissue Location Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.