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> Western Blot Stripping Buffer (Neutral)

Western Blot Stripping Buffer (Neutral)

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Referência E-IR-R102-100

Tamanho : 100mL

Marca : Elabscience

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Telefone : +1 850 650 7790

Product Introduction Western Blot Stripping Buffer is used for the reuse of the transferred protein membrane in Western blot. After primary antibody binding to the membrane and secondary antibody binding to the primary antibody, then using ECL subsequent to exposure the images, if the samples on the membrane are also needed to verify some control proteins or other target proteins, this product can be used to elute the combined antibodies before. After the antibody signals are cleared, the membrane can be re-incubated with control antibodies or other antibodies for the second verification. It can save the time of preparing SDS-PAGE gel and transfer, and the samples of experiment are also saved.
It only takes about 30 min to reuse the protein membrane which can eliminate the error caused by redoing the experiment and makes the experiment results more comparable.
Applications WB
Storage Store at 2~8°C for 12 months.
Shipping Biological ice pack at 4 ℃
Cautions 1. When the PVDF membrane is immersed with this product, the membrane will become transparent, which is a normal phenomenon. 2. In order to obtain the best performance, PVDF membrane is recommended for this product. 3. This product is recommended to ECL or similar chemiluminescent reagents. If non-chemiluminescent reagents, such as DAB is used to expose the image, please don’t use this product. 4. Repeated use of the same membrane with western blot experiments may lead to the weakening of protein signal. The membrane can be reused 1~2 times with several antibodies tests. 5. Repeated washing the PVDF membrane may lead to weak signal, please detect the lower expression protein first, and higher proteins such as control after cleaning with Western Blot Stripping Buffer. 6. For your safety and health, please wear experimental clothes and disposable gloves.
Other Clones

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Other Formats

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Biotin

Chromogenic Biotin

Cyanine 3

Cyanine 5

Cyanine 5.5

Cyanine 7

Elab Fluor®405

Elab Fluor®430

Elab Fluor®488

Elab Fluor®568

Elab Fluor®594

Elab Fluor®647

Elab Fluor®680

Elab Fluor®700

Elab Fluor®750

Elab Fluor®Red 780

Elab Fluor®Violet 450

FITC

HRP

NHS-Biotin

NHS-LC-LC-Biotin

Sulfo-NHS-Biotin

Sulfo-NHS-LC-LC-Biotin

Unconjugated

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Referência
Descrição
Cond.
Price Bef. VAT
150 500
Stripping Buffer
 500ml 
E-IR-R102-500
Western Blot Stripping Buffer (Neutral)
 500mL 
150 050
Stripping Buffer
 50ml